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苊对斯科特假丝酵母单倍体和二倍体培养物的诱变作用

[Mutagenic action of acenaphthene on haploid and diploid cultures of Candida scottii].

作者信息

Imshenetskiĭ A A, Kondrat'eva T F, Lin'kova M A

出版信息

Mikrobiologiia. 1985 May-Jun;54(3):360-2.

PMID:4046894
Abstract

Acenaphthene used as vapour increases the frequency of morphological mutations as well as mutations yielding strains with a reduced activity of respiration in Candida scottii. The diploid culture has more mutants than the haploid culture. The frequency of mutants depends on the duration of acenaphthene action. Cells more resistant to the action of acenaphthene vapour are selected in the haploid culture when C. scottii cells are transferred many times into new wort drops. As a result, the frequency of mutations decreases. When the diploid culture is exposed to the action of acenaphthene vapour for a longer time, the frequency of mutations rises. Apparently, the diploid culture has a lower activity of reparation of premutational damages than the haploid culture and therefore the number of mutant cells increases.

摘要

苊以蒸汽形式存在时,会增加斯科特假丝酵母形态突变的频率,以及产生呼吸活性降低菌株的突变频率。二倍体培养物中的突变体比单倍体培养物更多。突变体的频率取决于苊的作用持续时间。当斯科特假丝酵母细胞多次转移到新的麦芽汁滴中时,单倍体培养物中会筛选出对苊蒸汽作用更具抗性的细胞。结果,突变频率降低。当二倍体培养物长时间暴露于苊蒸汽作用时,突变频率会上升。显然,二倍体培养物修复突变前损伤的活性低于单倍体培养物,因此突变细胞的数量会增加。

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