Badten Alexander J, Oaxaca-Torres Susana, Basu Ritwika S, Gagnon Matthieu G, Torres Alfredo G
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77550.
Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX 77550.
Res Sq. 2025 May 13:rs.3.rs-6549424. doi: 10.21203/rs.3.rs-6549424/v1.
(), the etiological agent of melioidosis, lacks approved vaccines. However, several candidate vaccines have conferred protection in animal models. Interestingly, some of these vaccines have been shown to induce cross-protective immunity against the closely related species . This led us to explore whether there exists a subset of antigens that are conserved in other pathogenic species which could potentially serve as components of a pan- vaccine. We assessed the proteome of and identified several antigens that are conserved in the complex. To evaluate these antigens, we intranasally immunized mice with three proteins (OmpA1, OmpA2, Pal) coupled to an immunogenic gold nanoparticle (AuNP) platform, resulting in the induction of robust Th1/Th2-balanced responses and mucosal immunity. Animals immunized with AuNP-OmpA1 or AuNP-OmpA2 exhibited protection against a lethal respiratory challenge, which supports the idea that these antigens could be candidates for a cross-protective vaccine.
类鼻疽的病原体（）缺乏获批的疫苗。然而，几种候选疫苗已在动物模型中提供了保护。有趣的是，其中一些疫苗已被证明能诱导针对密切相关物种的交叉保护性免疫。这促使我们探索是否存在一组在其他致病物种中保守的抗原，这些抗原可能作为泛疫苗的组成成分。我们评估了的蛋白质组，并鉴定出在复合体中保守的几种抗原。为了评估这些抗原，我们用与免疫原性金纳米颗粒（AuNP）平台偶联的三种蛋白质（OmpA1、OmpA2、Pal）经鼻免疫小鼠，从而诱导出强烈的Th1/Th2平衡反应和黏膜免疫。用AuNP - OmpA1或AuNP - OmpA2免疫的动物在致死性呼吸道攻击中表现出保护作用，这支持了这些抗原可能是交叉保护性疫苗候选物的观点。
