Banjar Z M, Briggs R C, Hnilica L S, Stein J L, Stein G S
Mol Cell Biochem. 1985 Jul;67(2):101-10. doi: 10.1007/BF02370168.
Antisera to 0.35 M NaCl extracts and residues of S phase HeLa nuclei were reacted with electrophoretically separated proteins from the nuclei or nuclear material of HeLa cells synchronized in G1, S, G2 or M phases of the cell cycle. Quantitative evaluation of the peroxidase-antiperoxidase stained nitrocellulose transfers (Western blots) revealed significant changes in the quantities of nuclear non-histone proteins during the cell cycle. Immunochemical staining of electrophoretically separated nuclear antigens permits their selective detection in minute quantities and in the presence of many additional proteins.
将抗血清与处于细胞周期G1、S、G2或M期的同步化HeLa细胞核的0.35M NaCl提取物及残余物，与HeLa细胞核或核物质经电泳分离的蛋白质反应。对过氧化物酶-抗过氧化物酶染色的硝酸纤维素转移膜（蛋白质免疫印迹）进行定量评估，结果显示细胞周期中核内非组蛋白的量有显著变化。对经电泳分离的核抗原进行免疫化学染色，可以在微量以及存在许多其他蛋白质的情况下对其进行选择性检测。
