Budovskiĭ E I, Zalesskaia M A
Mol Biol (Mosk). 1985 Jul-Aug;19(4):1139-47.
It is shown that the action of beta-propiolactone on the bacteriophage MS2 under the wide range of conditions (pH, temperature, initial concentration of the reagent) the survival curves could be accurately described, taking into account the reagent consumption during inactivation. The requirements for accurate description of the infectivity inactivation of viruses by the action of any chemical agent were formulated. That allowed rational development of a principal stage in preparation of the killed antiviral vaccines--inactivation of the virus infectivity to the necessary extent by the action of chemical agents.
结果表明，在广泛的条件（pH值、温度、试剂初始浓度）下，β-丙内酯对噬菌体MS2的作用，考虑到灭活过程中试剂的消耗，存活曲线能够得到准确描述。阐述了任何化学试剂作用下准确描述病毒感染性灭活的要求。这使得灭活抗病毒疫苗制备主要阶段的合理开发成为可能——通过化学试剂的作用将病毒感染性灭活至必要程度。
