Budowsky E I, Friedman E A, Zheleznova N V
N.S. Zelinsky Institute of Organic Chemistry, USSR Academy of Sciences, Moscow.
Vaccine. 1991 Jul;9(7):473-6. doi: 10.1016/0264-410x(91)90031-z.
When the infectivity of the influenza virus is determined by means of titration on chicken embryos, calculating the infection titre according to Reed and Muench, the course of inactivation with beta-propiolactone shows an anomaly - the fraction of infected embryos in a batch initially increases and then decreases as the degree of dilution of the virus-containing sample is increased. This anomaly occurs because a slight dilution lowers the agent concentration insufficiently so that inactivation goes on after the dilution of the sample before and/or after the inoculation of the solution into the embryo. The anomaly can be avoided either by neutralizing or removing the agent prior to titration or by starting titration from high dilutions.
当通过在鸡胚上滴定来确定流感病毒的感染性，并根据里德和门奇方法计算感染滴度时，用β-丙内酯灭活的过程显示出一种异常现象——随着含病毒样品稀释度的增加，一批中感染胚胎的比例最初会增加，然后下降。出现这种异常现象是因为轻微稀释不足以降低试剂浓度，以至于在将溶液接种到胚胎之前和/或之后样品稀释后仍会继续灭活。可以通过在滴定前中和或去除试剂，或者从高稀释度开始滴定来避免这种异常现象。
