Sawyer J, Moore M M, Clive D, Hozier J
Mutat Res. 1985 Oct;147(5):243-53. doi: 10.1016/0165-1161(85)90065-2.
The cytogenetic characterization of the L5178Y TK+/-3.7.2C mouse lymphoma cell line was carried out, utilizing G-banded metaphase chromosomes, to provide a karyotypic basis for the precise delineation of induced rearrangements in TK-/- mutants. Band-pattern measurements were used to construct ideograms which represent the position, number, size and staining intensity of the chromosome bands. The TK+/-3.7.2C cell line has been shown to provide quantitation of forward mutations induced at the autosomal thymidine kinase (TK) locus in this cell line. Chromosome analysis of the TK+/-3.7.2C cell line and derived TK-/- mutants has become important in demonstrating that the TK+/-----TK-/- assay may detect and distinguish between chromosomal events and smaller, perhaps point-mutation, events in mutant colonies.
利用G带中期染色体对L5178Y TK+/-3.7.2C小鼠淋巴瘤细胞系进行了细胞遗传学特征分析，以便为精确描绘TK-/-突变体中的诱导重排提供核型基础。带型测量用于构建代表染色体带的位置、数量、大小和染色强度的 ideogram。TK+/-3.7.2C细胞系已被证明可对该细胞系常染色体胸苷激酶（TK）位点诱导的正向突变进行定量。对TK+/-3.7.2C细胞系及其衍生的TK-/-突变体进行染色体分析，对于证明TK+/-----TK-/-检测法可检测并区分突变菌落中的染色体事件和较小的、可能是点突变的事件具有重要意义。
