Hozier J, Sawyer J, Clive D, Moore M M
Mutat Res. 1985 Oct;147(5):237-42. doi: 10.1016/0165-1161(85)90064-0.
We have developed a cytogenetic technique that allows observation of chromosome rearrangements associated with TK-/- mutagenesis of the L5178Y/TK+/-3.7.2C cell line early in mutant clonal history. For a series of mutagenic treatments we show that the major proportion (93%) of small-colony (sigma) mutants studied have chromosome 11 rearrangements (the chromosome containing the thymidine kinase gene) while large-colony (lambda) mutants do not have detectable chromosome rearrangements. In addition, we find among the chromosome abnormalities in sigma mutants a significant proportion (34%) with dicentric chromosomes involving chromosome 11. These potentially unstable chromosome rearrangements may help to explain the karyotypic instability and heterogeneity among chromosome 11 aberrations previously noted in sigma mutants when they are analyzed later in their clonal history.
我们已经开发出一种细胞遗传学技术，该技术能够在突变克隆形成的早期观察到与L5178Y/TK+/-3.7.2C细胞系的TK-/-诱变相关的染色体重排。对于一系列诱变处理，我们发现，所研究的小集落（sigma）突变体中，大部分（93%）具有11号染色体重排（包含胸苷激酶基因的染色体），而大集落（lambda）突变体未检测到染色体重排。此外，我们发现在sigma突变体的染色体异常中，相当一部分（34%）具有涉及11号染色体的双着丝粒染色体。这些潜在不稳定的染色体重排可能有助于解释在sigma突变体克隆形成后期进行分析时，之前所观察到的11号染色体畸变之间的核型不稳定性和异质性。
