环戊烷和硝酸铵的遗传毒性评价。中国仓鼠卵巢细胞体外哺乳动物染色体畸变试验。
Evaluation of the Genetic Toxicity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells.
机构信息
Center for Chemical Safety and Health, Occupational Safety and Health Research Institute, Korea Occupational Safety and Health Agency, Daejeon, Korea.
出版信息
Saf Health Work. 2011 Mar;2(1):17-25. doi: 10.5491/SHAW.2011.2.1.17. Epub 2011 Mar 31.
OBJECTIVES
In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test.
METHODS
The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix).
RESULTS
Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method.
CONCLUSION
From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.
目的
本研究通过体外哺乳动物染色体畸变（CA）试验，进一步了解环戊烷和硝酸铵使用相关的危害。虽然这两种化学物质已经通过多种方法进行了测试，但尚未在 CA 试验中进行研究。
方法
采用卵巢幼稚细胞（CHO-K1 细胞），通过直接法（-S9）和代谢激活法（+S9 混合液）进行试验。
结果
直接法 48 小时处理组的 7 个剂量组，染色体畸变频率与剂量增加不成比例。代谢激活法 6 小时处理组，染色体畸变频率与剂量增加不成比例。
结论
根据这些发现，可以判定这两种化学物质在测试条件下不会引起染色体畸变。
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