Guo Xin-Long, Wu Xiang, Li Hong-Liang, Liu Ran-Xin, An Jian-Ping, You Chun-Xiang
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China; CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Hubei Hongshan Laboratory, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, China.
J Plant Physiol. 2025 Aug;311:154527. doi: 10.1016/j.jplph.2025.154527. Epub 2025 May 29.
Drought and salt stresses represent significant environmental constraints that severely impair global plant growth and development. While numerous transcription factors regulating drought and salt stress responses have been identified across plant species, their functional mechanisms remain incompletely understood. In this study, we characterized MdMYB62, a MYB transcription factor from apple (Malus domestica), and elucidated its functional role under abiotic stress conditions. Quantitative reverse transcription PCR (qRT-PCR) analysis revealed that MdMYB62 expression was significantly modulated under both drought and salt stress conditions. Functional analyses revealed that overexpression of MdMYB62 in apple calli led to increased sensitivity to drought and salt stress.Consistent with these findings, ectopic expression of MdMYB62 in Arabidopsis resulted in reduced tolerance to these stress, which was associated with elevated accumulation of reactive oxygen species (ROS). These results collectively establish MdMYB62 as a negative regulator of plant stress responses and provide new insights into the molecular mechanisms underlying plant adaptation to abiotic stress.
干旱和盐胁迫是严重影响全球植物生长发育的重要环境限制因素。虽然在多种植物中已鉴定出许多调控干旱和盐胁迫响应的转录因子，但其功能机制仍未完全清楚。在本研究中，我们对苹果（Malus domestica）的MYB转录因子MdMYB62进行了表征，并阐明了其在非生物胁迫条件下的功能作用。定量逆转录PCR（qRT-PCR）分析表明，MdMYB62的表达在干旱和盐胁迫条件下均受到显著调控。功能分析显示，MdMYB62在苹果愈伤组织中的过表达导致对干旱和盐胁迫的敏感性增加。与这些发现一致，MdMYB62在拟南芥中的异位表达导致对这些胁迫的耐受性降低，这与活性氧（ROS）积累增加有关。这些结果共同确立了MdMYB62作为植物胁迫响应的负调控因子，并为植物适应非生物胁迫的分子机制提供了新的见解。
