Department of Opthalmology, Gaziosmanpaşa Training and Research Hospital, İstanbul, Turkey.
Department of Opthalmology, Liv Hospital, Ankara, Turkey.
Eur J Ophthalmol. 2021 Mar;31(2):572-577. doi: 10.1177/1120672120902285. Epub 2020 Feb 5.
This research is aimed at determination of total oxidant status, total antioxidant status, serum thiol-disulfide, catalase, albumin, ischemia-modified albumin, and ceruloplasmin in patients suffering from retinitis pigmentosa and drawing a comparison with these parameters determined from healthy controls.
The study involved 35 patients of retinitis pigmentosa and 33 controls who were healthy individuals of comparable gender and age. Native thiol, total thiol, disulfide concentration, disulfide/native thiol, disulfide/total thiol, native thiol/total thiol ratios, total oxidant status, total antioxidant status, catalase, ceruloplasmin, albumin, and ischemia-modified albumin were determined from peripheral blood samples and comparison was drawn between the measurements of retinitis pigmentosa and controls.
The two groups were similar in gender and age distributions. It was found that retinitis pigmentosa group demonstrated greater total oxidant status, ischemia-modified albumin, and disulfide concentrations as compared to controls (p < 0.001). However, total antioxidant status, catalase, native thiol, total thiol, albumin, and ceruloplasmin of the two groups did not show statistically significant difference (p > 0.05). Moreover, disulfide/total thiol and disulfide/native thiol ratios of the retinitis pigmentosa group were significantly greater in comparison to controls (p < 0.05).
The researchers reached the conclusion that thiol oxidation in retinitis pigmentosa patients caused the dynamic thiol/disulfide homeostasis to shift toward the generation of disulfide. This is a novel research that involves analysis of thiol/disulfide homeostasis in retinitis pigmentosa patients using a novel automated assay. The researchers identified the cause for persistent oxidative stress and damage reported in retinitis pigmentosa patients. Still, future research is required for analysis of progression of antioxidant-oxidant state through various retinitis pigmentosa stages.
本研究旨在测定患有色素性视网膜炎患者的总氧化剂状态、总抗氧化状态、血清巯基-二硫键、过氧化氢酶、白蛋白、缺血修饰白蛋白和铜蓝蛋白，并与健康对照组的这些参数进行比较。
本研究纳入了 35 名色素性视网膜炎患者和 33 名健康对照组，两组患者的性别和年龄相匹配。从外周血样本中测定了天然巯基、总巯基、二硫键浓度、二硫键/天然巯基、二硫键/总巯基、天然巯基/总巯基比值、总氧化剂状态、总抗氧化状态、过氧化氢酶、铜蓝蛋白、白蛋白和缺血修饰白蛋白，并对色素性视网膜炎组和对照组的测量结果进行了比较。
两组患者在性别和年龄分布上相似。研究发现，与对照组相比，色素性视网膜炎组的总氧化剂状态、缺血修饰白蛋白和二硫键浓度更高（p<0.001）。然而，两组的总抗氧化状态、过氧化氢酶、天然巯基、总巯基、白蛋白和铜蓝蛋白差异无统计学意义（p>0.05）。此外，色素性视网膜炎组的二硫键/总巯基和二硫键/天然巯基比值明显高于对照组（p<0.05）。
研究人员得出结论，色素性视网膜炎患者的巯基氧化导致动态巯基/二硫键平衡向二硫键的生成方向移动。这是一项涉及使用新型自动化检测分析色素性视网膜炎患者巯基/二硫键平衡的新研究。研究人员确定了持续氧化应激和损伤在色素性视网膜炎患者中报道的原因。然而，仍需要进一步研究分析各种色素性视网膜炎阶段的抗氧化剂-氧化剂状态的进展。
