Njoroge Mary, Pinheiro Gabriela Pimentel, Santana Cinthia Vila Nova, Ali Hajar, Hobbs Stephanie, Mena-Bucheli Santiago, Romero-Sandoval Natalia, Robertson Steven, Rutter Charlotte E, Davoren Donna, Brooks Collin, Douwes Jeroen, Cooper Philip J, Mpairwe Harriet, Figueiredo Camila A, Cruz Alvaro A, Barreto Mauricio L, Pearce Neil, Pembrey Lucy
Department of Medical Statistics, London School of Hygiene and Tropical Medicine, London, UK.
ProAR Foundation and Federal University of Bahia, Salvador, Brazil.
Respir Res. 2025 Jun 5;26(1):212. doi: 10.1186/s12931-025-03279-6.
The Causes And MEchanisms foR non-atopic Asthma in children (CAMERA) study was designed to investigate risk factors and mechanisms of non-atopic asthma in children and young adults in Brazil, Ecuador, Uganda, and New Zealand. Initial epidemiological analyses using existing datasets identified and compared risk factors for both atopic and non-atopic asthma. The focus of this paper is the protocol for sample collection and analysis of clinical data on possible non-atopic mechanisms.
In each of the four centres, the CAMERA study will enroll 160 participants aged 10-28 years, equally distributed among atopic asthmatics (AA), non-atopic asthmatics (NAA), atopic non-asthmatics and non-atopic non-asthmatics. Participants will be new recruits or returning World ASthma Phenotypes (WASP) study participants. Phase I consists of skin prick tests to define atopy, a general CAMERA questionnaire that covers respiratory and general health to identify asthma cases, followed by an asthma control questionnaire for asthmatics only. Phase II consists of a stress questionnaire and the following clinical assessments: lung function, nasal cytology, blood sampling, in vitro whole blood stimulation to assess IFN-γ production, hair cortisol concentration, dry air and capsaicin challenges, plus in a subset, cold air challenges. Analyses will compare inflammatory, physiological and clinical parameters across the four groups overall and by country.
Here, we present the protocol for the CAMERA study, to provide relevant methodological details for CAMERA publications and to allow other centres globally to conduct similar analyses. The findings of this mechanistic multi-centre study will inform new and phenotype-specific prevention and treatment approaches.
Not applicable.
儿童非特应性哮喘的病因及机制（CAMERA）研究旨在调查巴西、厄瓜多尔、乌干达和新西兰儿童及青年非特应性哮喘的危险因素及机制。利用现有数据集进行的初步流行病学分析确定并比较了特应性和非特应性哮喘的危险因素。本文重点介绍样本采集方案以及对可能的非特应性机制临床数据的分析。
在四个中心的每个中心，CAMERA研究将招募160名年龄在10 - 28岁之间的参与者，他们将平均分配到特应性哮喘患者（AA）、非特应性哮喘患者（NAA）、特应性非哮喘患者和非特应性非哮喘患者中。参与者将是新招募的或之前参与过世界哮喘表型（WASP）研究的人员。第一阶段包括用于定义特应性的皮肤点刺试验、一份涵盖呼吸和整体健康状况以识别哮喘病例的通用CAMERA问卷，随后是仅针对哮喘患者的哮喘控制问卷。第二阶段包括一份应激问卷以及以下临床评估：肺功能、鼻细胞学检查、血液采样、用于评估IFN - γ产生的体外全血刺激试验、头发皮质醇浓度、干燥空气和辣椒素激发试验，另外在一个子集中还包括冷空气激发试验。分析将比较四组总体以及按国家划分的炎症、生理和临床参数。
在此，我们展示了CAMERA研究的方案，为CAMERA相关出版物提供相关方法学细节，并允许全球其他中心进行类似分析。这项多中心机制研究的结果将为新的和针对特定表型的预防及治疗方法提供依据。
不适用。
