Atiratana Tripatchara, Goldson Aliyah R, Samosorn Siritron, Rajput Neha, Praphairaksit Nalena, Kenney Justin W
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand 10110.
Department of Biological Sciences, Wayne State University, Detroit, MI, USA 48202.
bioRxiv. 2025 May 19:2025.05.16.654482. doi: 10.1101/2025.05.16.654482.
(L.) Aiton, commonly known as Arabian jasmine, is widely used in Thai traditional medicine for mental health ailments. While most studies in humans and animals find that Arabian jasmine reduces stress and anxiety, there are a handful of reports that it can oppose relaxation by increasing autonomic arousal. Using adult zebrafish, we sought to determine whether factors like strain, sex, and personality might contribute to the variable effects of on anxiety-related behavior. The flowers of were extracted by ultrasonic-assisted extraction with optimal air pressure. Headspace solid-phase microextraction with gas chromatography-mass spectrometry (HS-SPME-GC-MS) identified the main components in the Arabian jasmine flower extract, including linalool (an anxiolytic compound) and benzaldehyde. We fed three strains of zebrafish (AB, TL, and WIK) a gelatin pellet containing different concentrations of (5-20 mg kg) and assessed 3-dimensional swim behavior in the novel tank and mirror biting tests. We found that in female AB fish, resulted in a decrease in bottom distance during the novel tank test, consistent with an anxiogenic effect; there was no effect in WIK or TL fish. We also found that behavioral/personality type influenced the effects of where shy AB females increased their percent explored, consistent with an anxiolytic effect. Thus, we find that sex, genetics, and personality interact to influence the anxiety-related effects of Arabian jasmine suggesting that these factors may contribute to the opposing effects of Arabian jasmine reported in the literature.
素馨（L.）艾顿，通常被称为阿拉伯茉莉，在泰国传统医学中被广泛用于治疗精神健康疾病。虽然大多数对人类和动物的研究发现阿拉伯茉莉能减轻压力和焦虑，但也有少数报告称它可能通过增加自主神经兴奋来对抗放松。我们使用成年斑马鱼，试图确定品系、性别和个性等因素是否可能导致其对焦虑相关行为产生不同影响。采用最佳气压通过超声辅助提取法提取阿拉伯茉莉的花朵。顶空固相微萃取结合气相色谱 - 质谱联用（HS - SPME - GC - MS）鉴定了阿拉伯茉莉花提取物中的主要成分，包括芳樟醇（一种抗焦虑化合物）和苯甲醛。我们给三种品系的斑马鱼（AB、TL和WIK）投喂含有不同浓度阿拉伯茉莉（5 - 20毫克/千克）的明胶颗粒，并在新鱼缸试验和咬镜子试验中评估三维游泳行为。我们发现，在雌性AB品系斑马鱼中，阿拉伯茉莉在新鱼缸试验中导致底部停留距离减少，这与致焦虑作用一致；而在WIK或TL品系斑马鱼中没有影响。我们还发现行为/个性类型会影响阿拉伯茉莉的作用，其中害羞的AB品系雌性斑马鱼探索百分比增加，这与抗焦虑作用一致。因此，我们发现性别、基因和个性相互作用，影响阿拉伯茉莉与焦虑相关的作用，这表明这些因素可能导致了文献中报道的阿拉伯茉莉的相反作用。
