Croisier Marie, Busso Coralie, Kalbfuss Nils, Woglar Alexander, Knott Graham, Gönczy Pierre
Bio-EM platform, School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland.
MicroPubl Biol. 2025 May 22;2025. doi: 10.17912/micropub.biology.001624. eCollection 2025.
Centrioles are microtubule-based organelles important for cellular organization and function. embryos undergo extensive programmed centriole elimination, with merely 7 post-mitotic cells retaining a focus of the centriolar proteins SAS-4 and SAS-7 in L1 larvae. Here, we addressed whether such foci correspond to centrioles by conducting serial-section electron microscopy. Our analysis ascertains that centriolar microtubule configurations are indeed present in the B, F, U and Y rectal epithelial cells. Therefore, centrioles are truly spared from elimination in these cells, calling for investigating the importance of such retention compared to the elimination program occurring in most post-mitotic cells.
中心粒是基于微管的细胞器，对细胞的组织和功能很重要。胚胎经历广泛的程序性中心粒消除，在L1幼虫中仅有7个有丝分裂后细胞保留中心粒蛋白SAS-4和SAS-7的一个聚集点。在这里，我们通过进行连续切片电子显微镜分析来探讨这些聚集点是否对应于中心粒。我们的分析确定，在B、F、U和Y直肠上皮细胞中确实存在中心粒微管结构。因此，这些细胞中的中心粒确实未被消除，这就需要研究与大多数有丝分裂后细胞中发生的消除程序相比，这种保留的重要性。
