Robinson F, Petrig B L, Sinclair S H, Riva C E, Grunwald J E
Ophthalmology. 1985 Aug;92(8):1130-2. doi: 10.1016/s0161-6420(85)33896-4.
The acute effect of topical phenylephrine HCl 10%, tropicamide 1% and proparacaine HCl 0.5% on macular capillary blood flow was studied in six healthy human volunteers using the blue field simulation technique. This technique provides a method for quantifying the velocity of leukocytes flowing in one's own retinal macular capillaries. Patients adjusted the velocity of computer simulated leukocytes moving on a CRT screen to match that of their own entoptically perceived leukocytes before instillation of each of the tested preparations and for 35 minutes immediately thereafter A 5% NaCl solution was used as a control. We found no significant difference in leukocyte velocity between the control drop and any of the tested drugs. With the six subjects tested, the calculated average minimum percentage change in leukocyte velocity detectable with this technique (P less than 0.05, paired t-test) was 9%. None of the tested drugs affected macular leukocyte velocity, and presumably blood flow, by more than this amount.
采用蓝域模拟技术，在6名健康人类志愿者中研究了10%盐酸去氧肾上腺素、1%托吡卡胺和0.5%盐酸丙美卡因局部用药对黄斑区毛细血管血流的急性影响。该技术提供了一种量化自身视网膜黄斑区毛细血管中白细胞流动速度的方法。在滴注每种受试制剂之前及其后立即35分钟内，患者调整计算机模拟白细胞在阴极射线管屏幕上移动的速度，以使其与自身视内感知的白细胞速度相匹配。使用5%氯化钠溶液作为对照。我们发现对照滴眼液与任何一种受试药物之间白细胞速度无显著差异。在测试的6名受试者中，用该技术可检测到的白细胞速度计算平均最小百分比变化（P<0.05，配对t检验）为9%。受试药物均未使黄斑区白细胞速度及可能的血流受到超过该数值的影响。
