Sukhodub Leonid, Korenkov Oleksii, Sukhodub Liudmyla, Kumeda Mariia
Sumy State University, 116, Kharkivska Str. 2, Sumy, 40007, Ukraine.
Biomater Biosyst. 2025 May 4;18:100112. doi: 10.1016/j.bbiosy.2025.100112. eCollection 2025 Jun.
Given the problem of healing defects of the skull vault, this work is designed to compare in vivo on experimental animals the regenerative potential of osteogenic composite materials different in composition and properties: a) modified with nanostructured hydroxyapatite (HA) and Zn ions biopolymer alginate (Alg) matrix (Alg_HA), and b) loaded with nanoparticles (NPs) brushite (dicalcium phosphate dihydrate, DCPD) biopolymer chitosan (CS) matrix (CS_DCPD). Regeneration of the parietal bone defect in rats was studied on the 90th and 140th day using light and electron microscopy. Starting from the 90th day, mature lamellar bone tissue formation was observed in the peripheral parts of both implants, which was not yet detected in animals of the control group (healing under the blood clot). The materials underwent resorption during the experiment and were replaced by regenerated tissues. The complete biocompatibility of both materials and the absence of signs of inflammation have been proven. Alg_HA implantation contributed to faster formation and maturation of bone tissue compared to CS_DCPD.
鉴于颅骨穹窿愈合缺陷问题，本研究旨在对不同成分和性质的成骨复合材料在实验动物体内的再生潜力进行比较：a）用纳米结构羟基磷灰石（HA）和锌离子改性的生物聚合物海藻酸盐（Alg）基质（Alg_HA），以及b）负载纳米颗粒（NPs）的透钙磷石（磷酸二钙二水合物，DCPD）生物聚合物壳聚糖（CS）基质（CS_DCPD）。使用光学和电子显微镜在第90天和第140天对大鼠顶骨缺损的再生情况进行了研究。从第90天开始，在两种植入物的周边部分均观察到成熟的板层骨组织形成，而在对照组动物（血凝块下愈合）中尚未检测到。实验过程中材料发生吸收，并被再生组织替代。已证明两种材料均具有完全的生物相容性且无炎症迹象。与CS_DCPD相比，植入Alg_HA有助于骨组织更快地形成和成熟。
