He Zhijing, Jiao Chen, Wu Junnan, Gu Jiasen, Liang Huixin, Shen Lida, Yang Youwen, Tian Zongjun, Wang Changjiang, Jiang Qing
Institute of Additive Manufacturing (3D Printing), Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.
College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.
Int J Bioprint. 2023 Jan 13;9(2):668. doi: 10.18063/ijb.v9i2.668. eCollection 2023.
Porous hydroxyapatite (HA) scaffolds prepared by three-dimensional (3D) printing have wide application prospects owing to personalized structural design and excellent biocompatibility. However, the lack of antimicrobial properties limits its widespread use. In this study, a porous ceramic scaffold was fabricated by digital light processing (DLP) method. The multilayer chitosan/alginate composite coatings prepared by layer-by-layer method were applied to scaffolds and Zn was doped into coatings in the form of ion crosslinking. The chemical composition and morphology of coatings were characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Energy dispersive spectroscopy (EDS) analysis demonstrated that Zn was uniformly distributed in the coating. Besides, the compressive strength of coated scaffolds (11.52 ± 0.3 MPa) was slightly improved compared with that of bare scaffolds (10.42 ± 0.56 MPa). The result of soaking experiment indicated that coated scaffolds exhibited delayed degradation. o experiments demonstrated that within the limits of concentration, a higher Zn content in the coating has a stronger capacity to promote cell adhesion, proliferation and differentiation. Although excessive release of Zn led to cytotoxicity, it presented a stronger antibacterial effect against (99.4%) and (93%).
通过三维（3D）打印制备的多孔羟基磷灰石（HA）支架由于个性化的结构设计和优异的生物相容性而具有广阔的应用前景。然而，缺乏抗菌性能限制了其广泛应用。在本研究中，采用数字光处理（DLP）方法制备了一种多孔陶瓷支架。将通过层层法制备的多层壳聚糖/海藻酸盐复合涂层应用于支架，并以离子交联的形式将锌掺杂到涂层中。通过扫描电子显微镜（SEM）和X射线光电子能谱（XPS）对涂层的化学成分和形貌进行了表征。能量色散光谱（EDS）分析表明锌均匀分布在涂层中。此外，与裸支架（10.42±0.56 MPa）相比，涂层支架的抗压强度（11.52±0.3 MPa）略有提高。浸泡实验结果表明涂层支架表现出延迟降解。实验表明，在浓度范围内，涂层中较高的锌含量具有更强的促进细胞黏附、增殖和分化的能力。虽然过量释放的锌导致细胞毒性，但它对金黄色葡萄球菌（99.4%）和大肠杆菌（93%）表现出更强的抗菌效果。
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