Zhang Tian, Guo Chenxi, Yang Lu, Liu Lei, Sun Di, Long Xinqi, Wu Qi, Zhao Qiyang, Zhang Yaohai, Li Jing, Cui Yongliang, He Yue
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, P. R. China.
Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture and Rural Affairs, Citrus Research Institute, Southwest University, Chongqing, 400712, P. R. China.
Anal Methods. 2025 Jun 19;17(24):4935-4943. doi: 10.1039/d5ay00638d.
The detection of limonin, a bitter compound in citrus samples, is crucial for maintaining product quality, safety, and marketability and ultimately benefits both producers and consumers. In this assay to effectively modify the monoclonal antibody on gold nanobipyramids (AuNBPs), we replaced the original hexadecyltrimethylammonium bromide coating (CTAB) with SH-PEG2000-NHS. Then AuNBPs were modified with a limonin-specific monoclonal antibody (mAb) to create an AuNBP@mAb immunoprobe. We present a novel plasmonic AuNBP-based lateral flow immunoassay (LFIA) for colorimetric and photothermal assay of limonin. The limit of detection for the colorimetric mode of AuNBPs is 4.8 ng mL. The limit of detection for the photothermal mode of AuNBPs is 3.8 ng mL. Validation experiments using citrus samples demonstrate the robustness and reliability of the LFIA in real-world applications. Comparative analysis with ultra-high performance liquid chromatography tandem mass spectrometry confirms the accuracy of our LFIA.
检测柑橘样品中的苦味化合物柠檬苦素，对于维持产品质量、安全性和适销性至关重要，最终使生产者和消费者都受益。在本实验中，为了有效修饰金纳米双棱锥（AuNBPs）上的单克隆抗体，我们用SH-PEG2000-NHS取代了原来的十六烷基三甲基溴化铵涂层（CTAB）。然后用柠檬苦素特异性单克隆抗体（mAb）修饰AuNBPs，以创建AuNBP@mAb免疫探针。我们提出了一种基于等离子体AuNBP的新型侧向流动免疫分析（LFIA）方法，用于柠檬苦素的比色和光热分析。AuNBPs比色模式的检测限为4.8 ng/mL。AuNBPs光热模式的检测限为3.8 ng/mL。使用柑橘样品进行的验证实验证明了LFIA在实际应用中的稳健性和可靠性。与超高效液相色谱串联质谱法的对比分析证实了我们LFIA的准确性。
