Fabri João H T M, de Souza Layse C, Bergamo Luana W, Lynd Lee R, Olson Daniel G
Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, State of São Paulo, Brazil.
Terragia Biofuel Incorporated, Hanover, New Hampshire, USA.
J Bacteriol. 2025 Jul 24;207(7):e0010725. doi: 10.1128/jb.00107-25. Epub 2025 Jun 6.
is a promising candidate for the production of biofuels from lignocellulosic sugars; however, the genes associated with electron transfer from ferredoxin are poorly characterized. In this work, we deleted several key electron transfer genes. We showed that the gene is not necessary for high-yield ethanol production, but that a set of four other genes (, , , and ) are necessary. We showed that the gene can function as a monofunctional (i.e., non-bifurcating) FNOR enzyme in the absence of . The phenotypes of the , , and double-deletion strains are consistent with their function via hydrogen cycling.
The improved understanding of electron transfer pathways in will enable future efforts to transfer the robust ethanol production pathway from this microbe to other organisms, with potential implications for industrial biofuel production.
是从木质纤维素糖生产生物燃料的一个有前景的候选者；然而，与铁氧还蛋白电子转移相关的基因特征描述不足。在这项工作中，我们删除了几个关键的电子转移基因。我们表明该基因对于高产乙醇生产不是必需的，但另外一组四个基因（、、和）是必需的。我们表明在不存在的情况下，基因可以作为单功能（即非分叉）的FNOR酶发挥作用。、和双缺失菌株的表型与其通过氢循环的功能一致。
对中电子转移途径的更好理解将有助于未来将这种微生物强大的乙醇生产途径转移到其他生物体的努力，对工业生物燃料生产具有潜在意义。
