热厌氧菌糖解代谢工程方法。
Methods for Metabolic Engineering of Thermoanaerobacterium saccharolyticum.
机构信息
Thayer School of Engineering at Dartmouth, Hanover, NH, USA.
Center for Bioenergy Innovation, Oak Ridge, TN, USA.
出版信息
Methods Mol Biol. 2020;2096:21-43. doi: 10.1007/978-1-0716-0195-2_3.
PMID:32720144
Abstract
In this work, we describe genetic tools and techniques for engineering Thermoanaerobacterium saccharolyticum. In particular, the T. saccharolyticum transformation protocol and the methods for selecting for transformants are described. Methods for determining strain phenotypes are also presented.
摘要
在这项工作中，我们描述了用于工程化嗜热解糖梭菌的遗传工具和技术。特别是，描述了嗜热解糖梭菌的转化方案和转化子选择方法。还介绍了确定菌株表型的方法。
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