Vivanco-Maroto Santiaga María, Gómez-Marín Cristina, Recio Isidra, Miralles Beatriz
Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, Spain.
Escuela de Doctorado, Universidad Autónoma de Madrid, C. Francisco Tomás y Valiente, 2, 28049 Madrid, Spain.
Food Funct. 2025 Jul 1;16(13):5301-5311. doi: 10.1039/d5fo01394a.
The strong effect of protein digestion products on gastrointestinal hormone release is well recognized. However, little is known about the specific characteristics of hormone inducing peptides. A detailed analysis of food-derived peptides remaining in the human intestinal lumen after protein ingestion would constitute a practical strategy for the targeted identification of hormone inducing and DPP-IV inhibitory peptides. In this study, gastrointestinal resistant peptides derived from casein and whey protein were evaluated in epithelial intestinal cells. The secretion of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1) was evaluated in the STC-1 enteroendocrine cell line and the DPP-IV inhibitory potential was assayed using the Caco-2 cell line. Hydrophobic residues at N-terminal positions were crucial for the secretagogue and inhibitory activities, while the presence of multiple glutamic acid residues was shown to be a key trait for CCK secretion. The results appointed the sequence TPEVDDEALEKFDK from β-lactoglobulin as a strong CCK inducer. Additionally, KILDKVGINYWL, derived from α-lactalbumin, not only promoted GLP-1 secretion but also demonstrated significant DPP-IV inhibitory activity. These findings provide new insights into the functional potential of food-derived peptides, offering promising therapeutic avenues for regulating gastrointestinal hormones and improving metabolic health.
蛋白质消化产物对胃肠激素释放的强烈作用已得到充分认识。然而，关于激素诱导肽的具体特性却知之甚少。对蛋白质摄入后残留在人体肠腔内的食物源性肽进行详细分析，将构成一种有针对性地鉴定激素诱导肽和二肽基肽酶-IV（DPP-IV）抑制肽的实用策略。在本研究中，对源自酪蛋白和乳清蛋白的胃肠道抗性肽在上皮肠细胞中进行了评估。在STC-1肠内分泌细胞系中评估了胆囊收缩素（CCK）和胰高血糖素样肽-1（GLP-1）的分泌，并使用Caco-2细胞系测定了DPP-IV抑制潜力。N端位置的疏水残基对促分泌和抑制活性至关重要，而多个谷氨酸残基的存在被证明是CCK分泌的关键特征。结果确定β-乳球蛋白的序列TPEVDDEALEKFDK是一种强效的CCK诱导剂。此外，源自α-乳白蛋白的KILDKVGINYWL不仅促进了GLP-1的分泌，还表现出显著的DPP-IV抑制活性。这些发现为食物源性肽的功能潜力提供了新的见解，为调节胃肠激素和改善代谢健康提供了有前景的治疗途径。
