Zhao Chan, Xin Yu-Hua, Liu Qing
State Key Laboratory of Animal Biotech Breeding and College of Biological Sciences, China Agricultural University, Beijing 100193, P.R. China.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
Int J Syst Evol Microbiol. 2025 Jun;75(6). doi: 10.1099/ijsem.0.006799.
Three Gram-stain-negative, rod-shaped, motile bacterial strains, each with a single polar flagellum, designated LB3P38, LT1P18 and ZB1P45, were isolated from glacier samples on the Tibetan Plateau, P.R. China. These strains grew at temperatures ranging from -2 to 32 °C (optimum 25-30 °C) and at pH values of 5.0-11.0 (optimum pH 7.0). The similarities of 16S rRNA gene sequences among these strains ranged from 99.67% to 99.93%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed their affiliation with the genus . Phylogenomic analysis positioned these strains in close relation to PMCC 200367 and LMG 19851. Average nucleotide identity values and digital DNA-DNA hybridization values between these strains and other type strains of the genus were below 94.6% and 63.4%, respectively. The predominant fatty acids identified in these strains were C, C cyclo and summed feature 3 (C c and/or C c). Based on the combined phenotypic and phylogenetic evidence, each strain is proposed to represent a novel species within the genus , with the names sp. nov. (type strain=LB3 P38=CGMCC 1.11284=JCM 37126), sp. nov. (type strain=LT1 P18=CGMCC 1.11310=JCM 37131) and sp. nov. (type strain=ZB1 P45=CGMCC 1.23235=JCM 37141) proposed.
从中国青藏高原的冰川样本中分离出三株革兰氏阴性、杆状、具运动性的细菌菌株，每株都有一根单极鞭毛，分别命名为LB3P38、LT1P18和ZB1P45。这些菌株在-2至32°C（最适温度25 - 30°C）的温度范围内以及pH值为5.0 - 11.0（最适pH 7.0）的条件下生长。这些菌株之间16S rRNA基因序列的相似性在99.67%至99.93%之间。基于16S rRNA基因序列的系统发育分析证实它们属于该属。系统基因组分析表明这些菌株与PMCC 200367和LMG 19851关系密切。这些菌株与该属其他模式菌株之间的平均核苷酸同一性值和数字DNA - DNA杂交值分别低于94.6%和63.4%。在这些菌株中鉴定出的主要脂肪酸为C、C环以及总和特征3（C c和/或C c）。基于表型和系统发育的综合证据，提议将每株菌株代表该属内的一个新物种，分别命名为新种（模式菌株=LB3 P38=CGMCC 1.11284=JCM 37126）、新种（模式菌株=LT1 P18=CGMCC 1.11310=JCM 37131）和新种（模式菌株=ZB1 P45=CGMCC 1.23235=JCM 37141）。
