sp. nov. and sp. nov., isolated from wilted pepper plants.

Two Gram-stain-negative, aerobic, rod-shaped, non-endospore-forming bacteria, designated as strain MH1 and MH2, were isolated from branches of wilted pepper plants () collected from a farmland in Machong town, Guangdong, China, and investigated using a polyphasic approach. MH1 grew at temperatures of 4-42 °C (optimum 28 °C), with 0-6.0 % (w/v) NaCl and at pH 4.0-10.0 (optimum pH 4.0). MH2 grew at temperatures of 4-42 °C (optimum 28 °C), with 0-6.0% (w/v) NaCl and at pH 4.0-10.0 (optimum pH 5.0). Analysis of the 16S rRNA gene sequence indicated that MH1 belongs to and MH2 belongs to . Genome-based phylogenetic analysis further established that MH1 shares the closest evolutionary relationships with DSM 18929 and DSM 18941, and MH2 is sister to RW3S1. Whole-genome comparisons between MH1 and known species revealed average nucleotide identity (ANI) values up to 84.5%, as well as digital DNA-DNA hybridization (dDDH) values up to 28.3%, both substantially lower than the accepted thresholds for species delineation (ANI: 95%; dDDH: 70%). The ANI and dDDH values between MH2 and known species were at most 94.6 and 59.2 %, respectively. Additional biochemical and physiological analyses further support that MH1 and MH2 represent a novel species in and , respectively. Notably, the differences in carbon source utilization could differentiate MH1 and its close relatives in . The major fatty acids were iso-C, iso-C and iso-C for MH1 and were C, C ω7c/C ω6c (summed feature 8), C ω7c/C ω6c (summed feature 3) and C cyclo for MH2. Therefore, we propose a new species sp. nov., with MH1 (=GDMCC 1.3749=JCM 36317) as the type strain, and a new species sp. nov., with MH2 (=GDMCC 1.3750=JCM 36318) as the type strain. The MH1 genome has a size of 4.18 Mb and a GC-content of 67.19 mol%, while the MH2 genome has a size of 5.71 Mb and a GC-content of 63.12 mol%.