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用于X射线自由电子激光单粒子成像的同轴氦电喷雾

Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.

作者信息

Rafie-Zinedine Safi, Schulz Joachim, Bielecki Johan, Heymann Michael

机构信息

European XFEL, Holzkoppel 4, 22869 Schenefeld, Germany.

Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany.

出版信息

J Synchrotron Radiat. 2025 Jul 1;32(Pt 4):849-860. doi: 10.1107/S1600577525003686. Epub 2025 Jun 6.

DOI:10.1107/S1600577525003686
PMID:40478665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12236250/
Abstract

Single-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature. A coaxial liquid-sheet strategy considerably extended the range of suitable samples to include conductivities from zero to 40000 µS cm - a more than about an eightfold increase in range compared with conventional electrosprays. A helium chamber atmosphere in combination with an engineered gas-sheet protected aerosol formation against corona discharge and reduced background noise more than threefold. These results suggest new avenues to qualify ever more demanding biological and material science samples for single-particle imaging in the future.

摘要

在X射线自由电子激光下进行单粒子成像，依赖于在室温下将纳米级大分子和粒子以合适的方式注入气相。一种同轴液膜策略显著扩展了合适样品的范围，使其电导率涵盖从零到40000 μS/cm，与传统电喷雾相比，范围增加了约八倍多。氦气室气氛与设计的气膜相结合，保护气溶胶形成免受电晕放电影响，并将背景噪声降低了三倍多。这些结果为未来使要求更高的生物和材料科学样品符合单粒子成像条件提供了新途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/8769df21c62f/s-32-00849-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/50c557ba3ff6/s-32-00849-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/2b47e1c97865/s-32-00849-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/a6ab71540083/s-32-00849-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/63937acd5756/s-32-00849-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/7f17e123dbf0/s-32-00849-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/6df53d9498eb/s-32-00849-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/680205e15f47/s-32-00849-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/8769df21c62f/s-32-00849-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/50c557ba3ff6/s-32-00849-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/2b47e1c97865/s-32-00849-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/a6ab71540083/s-32-00849-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/63937acd5756/s-32-00849-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/7f17e123dbf0/s-32-00849-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/6df53d9498eb/s-32-00849-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/680205e15f47/s-32-00849-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30be/12236250/8769df21c62f/s-32-00849-fig8.jpg

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