Wang Yaxin, Zhong Zhiying, Ya Jinpan, Li Xinyuan, Yan Yan, Zhao Tiansheng, Li Na
School of Pharmacy, Jiangxi Medical College, Nanchang University, Key Laboratory of New Drug Transformation and Evaluation of Jiangxi Province, Nanchang 330031, PR China.
School of Pharmacy, Jiangxi Medical College, Nanchang University, Key Laboratory of New Drug Transformation and Evaluation of Jiangxi Province, Nanchang 330031, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 15;343:126497. doi: 10.1016/j.saa.2025.126497. Epub 2025 May 30.
The assessment of acid phosphatase (ACP) levels is of significant importance for evaluating a variety of physiological and pathological conditions, monitoring disease progression and guiding clinical therapy. We engineered a platform for ACP sensitive detection utilizing Prussian blue (PB)-incorporated heterometallic covalent organic framework (COF) nanozyme. The synthesized COF@PB/AuPt NPs demonstrate a synergistic enhancement in peroxidase-like catalytic activity originating from the combined action of PB and AuPt NPs. The introduction of COF as a support matrix endows the nanozyme with remarkable dispersity and stability, maintaining functionality even after prolonged storage for six months. The COF@PB/AuPt nanozyme can facilitate the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to oxidized TMB (oxTMB) under HO-rich conditions, which can be inhibited by reductive ascorbic acid (AA). Concurrently, ACP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate into AA, which can effectively inhibit the oxidation reaction of TMB. The sensitive assay for ACP has been successfully executed, achieving a remarkably low detection limit of 2.2 mU/L. The platform demonstrates exceptional selectivity against enzymatic interferents and robust analytical performance in complex biological matrices, as evidenced by recoveries of 95-107 % and RSD below 5 % in fetal bovine serum, underscoring its potential for clinical diagnostics and biomedical research.
酸性磷酸酶（ACP）水平的评估对于评估多种生理和病理状况、监测疾病进展以及指导临床治疗具有重要意义。我们构建了一个利用普鲁士蓝（PB）掺杂的异金属共价有机框架（COF）纳米酶进行ACP灵敏检测的平台。合成的COF@PB/AuPt NPs在类过氧化物酶催化活性方面表现出协同增强，这源于PB和AuPt NPs的联合作用。引入COF作为支撑基质赋予了纳米酶显著的分散性和稳定性，即使在长时间储存六个月后仍能保持功能。COF@PB/AuPt纳米酶在富含HO的条件下可促进3,3',5,5'-四甲基联苯胺（TMB）氧化为氧化型TMB（oxTMB），而还原型抗坏血酸（AA）可抑制该反应。同时，ACP催化L-抗坏血酸-2-磷酸水解为AA，AA可有效抑制TMB的氧化反应。已成功实现了对ACP的灵敏检测，检测限低至2.2 mU/L。该平台对酶干扰物具有出色的选择性，在复杂生物基质中具有稳健的分析性能，在胎牛血清中的回收率为95-107%，相对标准偏差低于5%，这突出了其在临床诊断和生物医学研究中的潜力。
