Wu Yingling, Liu Longxiao, Xu Xutao, Duan Yongmin, Fu Haojie, Wang Wenyu, Chen Zhijun, Liu Jinquan
Department of Health Inspection and Quarantine, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan, People's Republic of China.
Hunan Key Laboratory of Typical Environment Pollution and Health Hazards, University of South China, Hengyang, People's Republic of China.
Luminescence. 2025 Jul;40(7):e70257. doi: 10.1002/bio.70257.
Dopamine (DA) is a critical neurotransmitter linked to various neuropsychiatric disorders, emphasizing the need for precise detection in clinical settings. Herein, a novel colorimetric method for detecting DA was developed using gold nanocluster@covalent organic frameworks (AuNCs@COF) with peroxidase-like activity. The composite was created by integrating ultrasmall, uniformly distributed gold nanoclusters (AuNCs) into a COF matrix, retaining COF' advantages while enhancing catalytic activity and stability. AuNCs@COF catalyzed the oxidation of the colorless substrate TMB in the presence of HO, producing a blue product (oxTMB) with an absorbance peak at 652 nm. DA inhibited this catalytic activity, enabling visual and quantitative detection. The method demonstrated sensitivity (detection limit: 14 μmol/L) and a wide dynamic range (50-500 μmol/L), with excellent selectivity, reproducibility, and successful application in detecting DA in human urine samples (recovery rates: 93.7%-111.5%, RSD < 2.5%). This study underscores the potential of AuNCs@COF as a robust nanozyme for rapid, cost-effective, and user-friendly DA detection, holding promise for clinical diagnostics and point-of-care applications.
多巴胺（DA）是一种与多种神经精神疾病相关的关键神经递质，这凸显了在临床环境中进行精确检测的必要性。在此，利用具有过氧化物酶样活性的金纳米簇@共价有机框架（AuNCs@COF）开发了一种检测DA的新型比色法。该复合材料是通过将超小、均匀分布的金纳米簇（AuNCs）整合到COF基质中制成的，在保留COF优点的同时提高了催化活性和稳定性。在过氧化氢存在的情况下，AuNCs@COF催化无色底物TMB的氧化，产生在652nm处有吸收峰的蓝色产物（氧化型TMB）。DA抑制了这种催化活性，从而实现了可视化和定量检测。该方法具有灵敏度（检测限：14μmol/L）和宽动态范围（50 - 500μmol/L），具有出色的选择性、重现性，并成功应用于检测人尿液样本中的DA（回收率：93.7% - 111.5%，相对标准偏差<2.5%）。本研究强调了AuNCs@COF作为一种强大的纳米酶用于快速、经济高效且用户友好的DA检测的潜力，有望用于临床诊断和即时检测应用。
