Duarte Bianca Ferreira, Procopio Elisangela Dos Santos, de Matos Balsalobre Natália, da Rosa Guterres Zaira, Leite Kassuya Candida Aparecida, da Silva Pinto Luciano, Ferreira Moraes Carlos André, Soares Neto Raimundo Luciano, Nogueira Cláudio Rodrigo, Lima Cardoso Claudia Andrea
Center of Studies in Natural Resources, State University of Mato Grosso Do Sul (UEMS), Rodovia Dourados/Itahum, Km 12, Caixa Postal 364, 79804-970, Dourados, Mato Grosso do Sul, Brazil.
Graduate Program in Health Sciences, Federal University of Grande Dourados (UFGD), Rodovia Dourados/Itahum, Km 12, Caixa Postal 364, 79804-970, Dourados, Mato Grosso do Sul, Brazil.
J Ethnopharmacol. 2025 Jul 24;351:120097. doi: 10.1016/j.jep.2025.120097. Epub 2025 Jun 4.
Tarenaya aculeata is traditionally used in Brazil to treat inflammation and pain-related conditions.
This study aimed to investigate the chemical composition, anti-inflammatory potential, and genotoxicity of a freeze-dried stem decoction of T. aculeata (SE) and a fraction free of the bulk of water-soluble compounds (SF).
The SE and SF samples were chemically characterized using UHPLC-ESI-QTOF-MS/MS. The anti-inflammatory activity was evaluated at three doses (SE: 30, 100, and 300 mg/kg; SF: 10, 30, and 100 mg/kg) using carrageenan-induced paw edema and pleurisy models, while genotoxicity was assessed using the SMART test.
A total of 35 compounds were annotated: one cyanogenic glycoside; three glucosinolates; one benzoic acid derivative; one indole alkaloid; five spermidine alkaloids; six flavonoids; four sesquilignans; and fourteen miscellaneous compounds. Among them, seven new compounds: 4-cyano-4-hydroxybutanoic acid hexoside; tarenayanines A1, B1, C1, and D1; quercetin-sinapoyl-rhamnosyl-hexosyl-hexosyl-rhamnoside; and tarenayolone. SE and SF were not genotoxic and significantly reduced inflammatory parameters and pain sensitivity thresholds. The analgesic effect of SE was observed at 300 mg/kg, while those of SF at 100 mg/kg. SE significantly reduced edema at 100 and 300 mg/kg at 3 and/or 4 h post-induction, preventing mechanical hyperalgesia. SF inhibited edema only at 100 mg/kg, 3 and 4 h after administration. Both samples decreased leukocyte migration to pleural exudate significantly.
SE and SF showed in vivo anti-inflammatory action and were found to be rich in compounds associated with this therapeutic effect, supporting the use of T. aculeata for inflammation management.
在巴西，刺山柑传统上用于治疗炎症和疼痛相关病症。
本研究旨在调查刺山柑冻干茎煎剂（SE）和去除大部分水溶性化合物的馏分（SF）的化学成分、抗炎潜力和遗传毒性。
使用超高效液相色谱-电喷雾电离-四极杆飞行时间串联质谱（UHPLC-ESI-QTOF-MS/MS）对SE和SF样品进行化学表征。使用角叉菜胶诱导的爪肿胀和胸膜炎模型，在三个剂量（SE：30、100和300mg/kg；SF：10、30和100mg/kg）下评估抗炎活性，同时使用体细胞诱变反应试验（SMART试验）评估遗传毒性。
共鉴定出35种化合物：一种氰苷；三种硫代葡萄糖苷；一种苯甲酸衍生物；一种吲哚生物碱；五种亚精胺生物碱；六种黄酮类化合物；四种倍半木脂素；以及十四种其他化合物。其中，七种新化合物：4-氰基-4-羟基丁酸己糖苷；刺山柑碱A1、B1、C1和D1；槲皮素-芥子酰基-鼠李糖基-己糖基-己糖基-鼠李糖苷；以及刺山柑酮。SE和SF无遗传毒性，并显著降低炎症参数和疼痛敏感性阈值。SE在300mg/kg时观察到镇痛作用，而SF在100mg/kg时观察到镇痛作用。SE在诱导后3小时和/或4小时，在100和300mg/kg时显著减轻肿胀，预防机械性痛觉过敏。SF仅在给药后3小时和4小时，在100mg/kg时抑制肿胀。两种样品均显著降低白细胞向胸腔渗出液的迁移。
SE和SF显示出体内抗炎作用，并且发现富含与这种治疗作用相关的化合物，支持刺山柑用于炎症管理。
