Jha Prabhat Kumar, Pandey Bipindra, Yadav Ram Kishor, Sindhu K C, Poudel Sandesh, Panta Sushil
Department of Pharmacy, Madan Bhandari Academy of Health Sciences, Hetauda, Nepal.
School of Health and Allied Sciences, Pokhara University, Pokhara, Nepal.
PLoS One. 2025 Jun 23;20(6):e0326808. doi: 10.1371/journal.pone.0326808. eCollection 2025.
Aleuritopteris bicolor (Roxb.) Fraser-Jenk. is used in traditional medicine in Nepal to treat a variety of ailments. This study aimed to investigate qualitative phytochemical screening, quantitative phytochemical analysis (total phenolic and flavonoid content estimation), in vitro antioxidant activity, alpha-amylase inhibitory activity of Aleuritopteris bicolor (Roxb.) Fraser-Jenk. leaves from Nepal. Furthermore, this study examined the analgesic (tail flick and hot plate test method) and anti-inflammatory (carrageenan-induced paw edema) activities of Aleuritopteris bicolor leaves in an acute pain and inflammation rat model. The Aleuritopteris bicolor extract exhibited flavonoid (405.95 ± 0.28 mg QE/g) and phenolic content (20.98 ± 0.20 mg GAE/g). Aleuritopteris bicolor showed free radical scavenging activity with IC50 value in difference antioxidant methods (IC50:9.87 µg/mL, DPPH scavenging assay; 249.59 µg/mL, Hydrogen peroxide; 72.98 µg/mL, Nitric oxide assay), and significant Ferric reducing power assay (0.51 ± 0.004). It increased analgesic activity by 38.89% (hot plate test) and 16.84% (tail-flick test) at 250 mg/kg, reduced inflammation by 36.44% at 180 min (500 mg/kg), and inhibited alpha-amylase activity (IC50:59.31 µg/mL). Aleuritopteris bicolor exhibits significant analgesic, anti-inflammatory, and hypoglycemic potential, making it a promising candidate for further pharmacological exploration. This study marks the first scientific study of the in vivo analgesic and anti-inflammatory effects from the leaf extract of Aleuritopteris bicolor till date.
双色金粉蕨（Aleuritopteris bicolor (Roxb.) Fraser-Jenk.）在尼泊尔传统医学中用于治疗多种疾病。本研究旨在对来自尼泊尔的双色金粉蕨叶片进行定性植物化学筛选、定量植物化学分析（总酚和黄酮含量测定）、体外抗氧化活性、α-淀粉酶抑制活性研究。此外，本研究还采用急性疼痛和炎症大鼠模型，检测了双色金粉蕨叶片的镇痛（甩尾和热板试验法）和抗炎（角叉菜胶诱导的爪肿胀）活性。双色金粉蕨提取物的黄酮含量为（405.95±0.28 mg QE/g），酚含量为（20.98±0.20 mg GAE/g）。双色金粉蕨在不同抗氧化方法中均表现出自由基清除活性，其IC50值分别为：DPPH清除试验9.87 μg/mL、过氧化氢清除试验249.59 μg/mL、一氧化氮清除试验72.98 μg/mL，同时具有显著的铁还原能力（0.51±0.004）。在250 mg/kg剂量下，其镇痛活性在热板试验中提高了38.89%，在甩尾试验中提高了16.84%；在500 mg/kg剂量下，180分钟时炎症减轻了36.44%；对α-淀粉酶活性的抑制IC50值为59.31 μg/mL。双色金粉蕨具有显著的镇痛、抗炎和降血糖潜力，是进一步药理研究的有前景的候选植物。本研究是迄今为止对双色金粉蕨叶提取物体内镇痛和抗炎作用的首次科学研究。
