Kronenberg Katharina, Dormann Frauke, Brosig Andreas, Pamler Irene, Geissler Edward K, Burkhardt Ralph, Offner Robert, Hutchinson James A, Haehnel Viola
Department of Surgery, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
Institute of Clinical Chemistry and Laboratory Medicine, Transfusion Medicine, University Hospital Regensburg, Regensburg, Germany.
Sci Rep. 2025 Jun 6;15(1):19923. doi: 10.1038/s41598-025-04350-4.
Human leukocyte concentrates recovered from leukocyte reduction system (LRS) chambers of the Trima Accel automated blood collection device are a by-product of platelet apheresis that is often used in research. The Trima Accel software was obligatorily updated in 2023 from version 6 to 7. Here, we investigated software-dependent differences in the cellular composition of LRS concentrates when performing apheresis with either plasma or platelet additive solution. When using plasma as suspension medium, the software update to Trima 7 led to higher fraction of B cells as revealed by flow cytometry. Compared to platelets-in-plasma collection, the total recovered volume and leukocyte density was significantly reduced when running Trima 7 software with platelet additive solution including a plasma rinseback. Moreover, the proportion of lymphocytes and monocytes in these products was lower, whereas the proportion of neutrophils and eosinophils was higher. Researchers working with leukocytes isolated from LRS chambers should be aware that performing apheresis with platelet additive solution alters the composition of their starting material, which could affect the interpretation of some experiments.
从Trima Accel自动血液采集设备的白细胞去除系统（LRS）腔室中回收的人白细胞浓缩物是血小板单采的副产品，常用于研究。Trima Accel软件在2023年强制从版本6更新到版本7。在此，我们研究了在使用血浆或血小板添加剂溶液进行单采时，LRS浓缩物细胞组成中与软件相关的差异。当使用血浆作为悬浮介质时，流式细胞术显示软件更新到Trima 7会导致B细胞比例更高。与血浆中血小板采集相比，在运行Trima 7软件并使用包括血浆回冲的血小板添加剂溶液时，总回收体积和白细胞密度显著降低。此外，这些产品中淋巴细胞和单核细胞的比例较低，而中性粒细胞和嗜酸性粒细胞的比例较高。使用从LRS腔室分离的白细胞进行研究的人员应注意，使用血小板添加剂溶液进行单采会改变起始材料的组成，这可能会影响某些实验的解释。
