Leo M A, Mak K M, Savolainen E R, Lieber C S
Proc Soc Exp Biol Med. 1985 Nov;180(2):382-91. doi: 10.3181/00379727-180-42193.
Successful isolation and culture of myofibroblasts from rat liver is described for the first time. After collagenase digestion of livers, obtained from rats fed regular laboratory chow diet as well as control and vitamin A-supplemented liquid diets, cells were separated on a Percoll density gradient. Cells characterized as myofibroblasts by electron microscopy were cultured in a Dulbecco's modified Eagle's medium. Characteristic features of myofibroblasts (abundant myofibrils) with dense bodies, indented nucleus, basal lamina-like structure, capacity to synthesize types I, III, and IV collagens and laminin were demonstrated in both primary and secondary cultures.
首次描述了从大鼠肝脏成功分离和培养肌成纤维细胞的方法。在用胶原酶消化从喂食常规实验室饲料以及对照和补充维生素A的液体饲料的大鼠获得的肝脏后，细胞在Percoll密度梯度上进行分离。通过电子显微镜鉴定为肌成纤维细胞的细胞在杜氏改良 Eagle 培养基中培养。在原代和传代培养中均证实了肌成纤维细胞的特征性特征（丰富的肌原纤维），包括致密小体、凹陷的细胞核、基底膜样结构、合成I、III和IV型胶原蛋白以及层粘连蛋白的能力。
