Friberger P
Prog Clin Biol Res. 1985;189:139-49.
An endotoxin assay utilizing Limulus lysate (LAL) and a chromogenic peptide substrate is described. The assay of picograms of a compound, present in significant quantities almost everywhere, is obviously associated with a serious risk of contamination. Measures to avoid and detect contamination have been considered. When a quantitative assay like the present one is used, the inhibitory effects of samples analyzed are obvious and also possible to quantify. Methods to avoid such inhibitory effects have been studied and the simplest procedure seems to be dilution with endotoxin-free water, in some cases also combined with heat treatment (75 degrees C for 5 min).
描述了一种利用鲎试剂（LAL）和显色肽底物的内毒素检测方法。对几乎在任何地方都大量存在的化合物进行皮克级别的检测，显然存在严重的污染风险。已经考虑了避免和检测污染的措施。当使用像本方法这样的定量检测时，所分析样品的抑制作用很明显，而且也可以进行量化。已经研究了避免这种抑制作用的方法，最简单的程序似乎是用无内毒素水稀释，在某些情况下还可结合热处理（75℃，5分钟）。
