Lichtensteiger W, Schlumpf M
Pharmacol Biochem Behav. 1985 Sep;23(3):439-44. doi: 10.1016/0091-3057(85)90018-8.
In order to study effects of nicotine on fetal gonadal axis and sexually dimorphic behavior, time-pregnant Sprague Dawley rats were implanted on gestational day (GD) 12 with an osmotic minipump containing either nicotine tartrate, tartaric acid or saline. Others were sham-operated on GD 12 or left untreated. Male fetuses of all control groups displayed the characteristic rise in plasma testosterone at GD 18 (as compared to GD 17 and 19); this was abolished by nicotine. Adult offspring of untreated or tartaric acid-treated dams exhibited a marked sexual dimorphism in their preference for saccharin-containing drinking water at 0.06-0.25%. No such sex difference was seen in offspring of nicotine-treated rats. In controls, the sexes differed with respect to the proportion of rats with high saccharin preference. In the group of males prenatally exposed to nicotine, the proportion of animals with high preference increased to the female level. These data indicate that prenatal exposure to nicotine can interfere with the development of the male gonadal axis and with the organization of sexually dimorphic behavior.
为了研究尼古丁对胎儿性腺轴和性别二态性行为的影响，在妊娠第12天（GD12），将怀孕的斯普拉格-道利大鼠植入含有酒石酸尼古丁、酒石酸或生理盐水的渗透微型泵。其他大鼠在GD12进行假手术或不进行处理。所有对照组的雄性胎儿在GD18时血浆睾酮均呈现特征性升高（与GD17和19相比）；而尼古丁消除了这种升高。未处理或酒石酸处理的母鼠的成年后代在对含0.06 - 0.25%糖精的饮用水的偏好上表现出明显的性别二态性。在尼古丁处理的大鼠后代中未观察到这种性别差异。在对照组中，大鼠对高糖精偏好的比例存在性别差异。在产前暴露于尼古丁的雄性组中，具有高偏好的动物比例增加到了雌性水平。这些数据表明，产前暴露于尼古丁会干扰雄性性腺轴的发育以及性别二态性行为的组织。
