Tao Zhenxiang, Sun Yue, Zhou Mengxiao, Li Tengteng, Lan Weiqing, Zhao Yong, Sun Xiaohong
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, People's Republic of China; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, People's Republic of China.
Int J Food Microbiol. 2025 Oct 2;441:111303. doi: 10.1016/j.ijfoodmicro.2025.111303. Epub 2025 Jun 4.
Vibrio parahaemolyticus poses a serious threat to the aquaculture industry and human health. Two novel phages (vB_VpaS_1601 and vB_VpaP_1701) were isolated from oysters. Phage vB_VpaS_1601 and phage vB_VpaP_1701 exhibited short latent periods and high burst sizes. In addition, both phages showed stability across a broad range of temperature and pH conditions. Genomic analyses revealed that phage vB_VpaS_1601 is a novel genus of the Caudoviricetes class, and vB_VpaP_1701 is a new species of the Maculvirus genus, Autographiviridae family. Both single phage and phage cocktail can inhibit V. parahaemolyticus proliferation, with phage cocktail (MOI = 1) can completely inhibit the growth of V. parahaemolyticus within 12 h. The phages reduced biofilm formation by 53.57 % (vB_VpaS_1601) and 64.88 % (vB_VpaP_1701), while the cocktail achieved 46 %-78 % inhibition. Phage cocktail can reduce V. parahaemolyticus by 1.53-2.74 log CFU/cm in salmon and 1.56-2.91 log CFU/cm in oysters. These results indicated that vB_VpaS_1601 and vB_VpaP_1701 have potential as antibacterial agents for controlling V. parahaemolyticus in seafood products.
副溶血性弧菌对水产养殖业和人类健康构成严重威胁。从牡蛎中分离出两种新型噬菌体（vB_VpaS_1601和vB_VpaP_1701）。噬菌体vB_VpaS_1601和噬菌体vB_VpaP_1701表现出潜伏期短和裂解量高的特点。此外，两种噬菌体在广泛的温度和pH条件下都表现出稳定性。基因组分析表明，噬菌体vB_VpaS_1601是尾病毒目（Caudoviricetes）的一个新属，vB_VpaP_1701是自动病毒科（Autographiviridae）黄斑病毒属（Maculvirus）的一个新种。单一噬菌体和噬菌体鸡尾酒都能抑制副溶血性弧菌的增殖，噬菌体鸡尾酒（感染复数=1）能在12小时内完全抑制副溶血性弧菌的生长。噬菌体使生物膜形成减少了53.57%（vB_VpaS_1601）和64.88%（vB_VpaP_1701），而噬菌体鸡尾酒的抑制率为46%-78%。噬菌体鸡尾酒可使鲑鱼中的副溶血性弧菌减少1.53-2.74 log CFU/cm，使牡蛎中的副溶血性弧菌减少1.56-2.91 log CFU/cm。这些结果表明，vB_VpaS_1601和vB_VpaP_1701作为控制海产品中副溶血性弧菌的抗菌剂具有潜力。
