Liu Yingli, Wu Kunrun, Jiao Lingxia, Shen Rui, Ran Junjian, Wu Youzhi
School of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China.
Institute of Biomaterials and Biomedicine, School of Food and Pharmacy, Shanghai Zhongqiao Vocational and Technical University, Shanghai, 201514, China.
Appl Microbiol Biotechnol. 2025 Jun 7;109(1):141. doi: 10.1007/s00253-025-13525-y.
Alicyclobacillus acidoterrestris causes pasteurized acidic juices spoilage, resulting in a significant decline in juice quality and causing economic losses. Exploration of A. acidoterrestris in response to acid stress could help control contamination caused by the bacteria. In this study, the mechanism of A. acidoterrestris in response to acid stress was studied by quantitative phosphoproteomics technique. Results showed that the phosphorylation of 40 proteins in A. acidoterrestris was closely related to the regulation of acid stress. The KEGG pathway enrichment analysis showed that the quorum sensing pathway, which might be involved in the perception of A. acidoterrestris, was mainly enriched. We found that the upregulation of Spo0A and YidC phosphorylationmay resist acid stress by forming spores. The phosphorylation level of pyruvate kinase increased, which may improve bacterial acid stress resistance through the formation of energy supply. The phosphorylation level of ABC transporter permease was significantly upregulated, which may be part of the cell adaptation adjustment and contribute to the survival of A. acidoterrestris under acid stress. In summary, the molecular mechanism of acid stress regulation of A. acidoterrestris was proposed via quantitative phosphoproteomics, which provided a theoretical and experimental basis for further investigation of the acid resistance mechanism of A. acidoterrestris. KEY POINTS: • Spo0 A, Yidc proteins may be the key regulatory proteins for acid stress response. • ABC transporters are beneficial to the survival of the bacteria under acidic stress. • A. acidoterrestris may sense and transmit pH signal through quorum sensing pathway.
嗜酸耐热芽孢杆菌会导致巴氏杀菌酸性果汁变质，致使果汁品质大幅下降并造成经济损失。探究嗜酸耐热芽孢杆菌对酸胁迫的响应有助于控制该细菌引起的污染。在本研究中，采用定量磷酸化蛋白质组学技术研究了嗜酸耐热芽孢杆菌对酸胁迫的响应机制。结果表明，嗜酸耐热芽孢杆菌中40种蛋白质的磷酸化与酸胁迫调控密切相关。KEGG通路富集分析表明，可能参与嗜酸耐热芽孢杆菌感知的群体感应通路主要被富集。我们发现，Spo0A和YidC磷酸化的上调可能通过形成芽孢来抵抗酸胁迫。丙酮酸激酶的磷酸化水平升高，这可能通过形成能量供应来提高细菌的抗酸胁迫能力。ABC转运蛋白通透酶的磷酸化水平显著上调，这可能是细胞适应性调节的一部分，并有助于嗜酸耐热芽孢杆菌在酸胁迫下存活。综上所述，通过定量磷酸化蛋白质组学提出了嗜酸耐热芽孢杆菌酸胁迫调控的分子机制，为进一步研究嗜酸耐热芽孢杆菌的抗酸机制提供了理论和实验依据。要点：• Spo0A、Yidc蛋白可能是酸胁迫响应的关键调节蛋白。• ABC转运蛋白有利于细菌在酸性胁迫下存活。• 嗜酸耐热芽孢杆菌可能通过群体感应通路感知并传递pH信号。
