Ding Mengyu, Zhou Bokai, Piao Junjie, Cai Aijun, Jin Mingshi
Department of Chemistry (College of Science), Yanbian University, Yanji, Jilin 133002, China; Hebei Key Laboratory of Ocean Dynamics, Resources and Environments, Hebei Normal University of Science & Technology, Qinhuangdao 066004, China.
Department of Chemistry (College of Science), Yanbian University, Yanji, Jilin 133002, China.
Colloids Surf B Biointerfaces. 2025 Oct;254:114863. doi: 10.1016/j.colsurfb.2025.114863. Epub 2025 Jun 5.
Herein, we constructed a near-infrared (NIR) responsive CuS/CuO cascade nanozyme system addressing the critical limitation of exogenous HO dependency in conventional therapies. This system incorporates NIR-responsive antibacterial CuS/CuO composite based on cascade nanoenzymes. The loaded CuO nanoparticles (NPs) spontaneously generate Cu and HO in situ upon NIR irradiation (808 nm). This initiates a dual catalytic pathway: (1) Cu-mediated Fenton-like reactions and (2) HO-activated peroxidase-like activity, synergistically producing ·OH radicals (EPR-confirmed). Under NIR irradiation, CuO releases HO that activates the peroxidase-like activity of CuS. During the following cascade reaction, Cu catalyzes a Fenton-like reaction to produce a large amount of ·OH, while HO activates peroxidase-like activity in the CuS/CuO composite. Additionally, CuS/CuO also exhibits oxidase-like activity. The oxidase-like activity coupled with the cascade reaction simulated with photothermal conditions allows CuS/CuO (100 μg/mL) to effectively destroy Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Methicillin-resistant Staphylococcus aureus (MRSA). Moreover, the CuS/CuO composite effectively inhibits the formation of biofilms of E. coli, S. aureus, and MRSA. ·OH was confirmed to be the main free radical during the antimicrobial process of CuS/CuO. In vivo anti-infection assays prove that CuS/CuO could accelerate wound healing under NIR irradiation with negligible toxicity. This self-sustaining system eliminates exogenous HO requirements while achieving combinatorial antimicrobial efficacy through photothermal-potentiated nanozyme cascades.
在此，我们构建了一种近红外（NIR）响应的CuS/CuO级联纳米酶系统，以解决传统疗法中对外源过氧化氢（HO）依赖的关键限制。该系统包含基于级联纳米酶的近红外响应抗菌CuS/CuO复合材料。负载的氧化铜纳米颗粒（NPs）在近红外辐射（808 nm）下原位自发产生铜离子（Cu）和过氧化氢（HO）。这引发了一条双催化途径：（1）铜介导的类芬顿反应和（2）过氧化氢激活的类过氧化物酶活性，协同产生羟基自由基（·OH，经电子顺磁共振证实）。在近红外辐射下，氧化铜释放过氧化氢，激活硫化铜的类过氧化物酶活性。在随后的级联反应中，铜催化类芬顿反应产生大量的·OH，而过氧化氢激活CuS/CuO复合材料中的类过氧化物酶活性。此外，CuS/CuO还表现出类氧化酶活性。类氧化酶活性与光热条件模拟的级联反应相结合，使得CuS/CuO（100μg/mL）能够有效破坏大肠杆菌（E. coli）、金黄色葡萄球菌（S. aureus）和耐甲氧西林金黄色葡萄球菌（MRSA）。此外，CuS/CuO复合材料有效抑制了大肠杆菌、金黄色葡萄球菌和MRSA生物膜的形成。·OH被证实是CuS/CuO抗菌过程中的主要自由基。体内抗感染试验证明，CuS/CuO在近红外辐射下可加速伤口愈合，且毒性可忽略不计。这种自我维持的系统消除了对外源过氧化氢的需求，同时通过光热增强的纳米酶级联反应实现了联合抗菌效果。
