Tan Xiaofeng, Lin Nanyun, Yang Sha, Gong Hongyu, Wang Minghui, Li Na, Liu Fen, Rao Dajun, Wu Yingying, Tang Jing, Yang Qinglai
Department of Anesthesiology, Hunan Provincial Maternal and Child Health Care Hospital & NHC Key Laboratory of Birth Defect Research and Prevention & MOE Key Lab of Rare Pediatric Disease, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital & Center for Molecular Imaging Probe, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Adv Sci (Weinh). 2025 Jul;12(27):e2502391. doi: 10.1002/advs.202502391. Epub 2025 Apr 27.
Diabetic wound healing presents serious clinical challenges due to the unique wound microenvironment characterized by hyperglycemia, bacterial infection, excessive oxidative stress, and hypoxia. Herein, a copper peroxide (CuO)-coated AuCu bimetallic aerogel is developed that exhibits quadruple enzyme-mimicking activity and HO/O self-supplying to modulate the complex microenvironment of methicillin-resistant staphylococcus aureus (MRSA)-infected diabetic wounds. The AuCu@CuO aerogels demonstrate favorable photothermal properties and mimic four enzyme-like activities: peroxidase-like activity for producing toxic reactive oxygen species; catalase-like activity for decomposing HO to release O to relieve oxidative stress and hypoxia; glucose oxidase-like activity for reducing excessive blood glucose and glutathione peroxidase-like activity for balancing abnormal glutathione level. The CuO coating facilitates a continuous and adequate in situ production of HO within the mildly acidic infection microenvironment, enabling excellent antibacterial activity and reduced blood glucose levels during the initial treatment of infected diabetic wounds. Furthermore, the engineered AuCu@CuO aerogels not only scavenge elevated ROS during the inflammatory phase but also synergistically generate oxygen to promote wound healing. Overall, the AuCu@CuO aerogelsmicroenvironment can be activated by the diabetic wound infection microenvironments, alleviating inflammation, reducing hypoxia, lowering blood glucose levels, and enhancing angiogenesis and collagen fiber accumulation, thereby significantly improving diabetic wound healing.
由于糖尿病伤口愈合的独特微环境具有高血糖、细菌感染、过度氧化应激和缺氧等特征，因此带来了严峻的临床挑战。在此，我们开发了一种过氧化铜（CuO）包覆的金铜双金属气凝胶，它具有四重酶模拟活性和HO/O自供应能力，可调节耐甲氧西林金黄色葡萄球菌（MRSA）感染的糖尿病伤口的复杂微环境。AuCu@CuO气凝胶具有良好的光热性能，并模拟四种酶样活性：过氧化物酶样活性，用于产生活性氧；过氧化氢酶样活性，用于分解HO以释放O以减轻氧化应激和缺氧；葡萄糖氧化酶样活性，用于降低过高的血糖；谷胱甘肽过氧化物酶样活性，用于平衡异常的谷胱甘肽水平。CuO涂层有助于在轻度酸性感染微环境中原位持续且充分地产生HO，在感染性糖尿病伤口的初始治疗期间具有出色的抗菌活性并降低血糖水平。此外，经过工程设计的AuCu@CuO气凝胶不仅在炎症阶段清除升高的ROS，还能协同产生氧气以促进伤口愈合。总体而言，AuCu@CuO气凝胶微环境可被糖尿病伤口感染微环境激活，减轻炎症、减少缺氧、降低血糖水平并增强血管生成和胶原纤维积累，从而显著改善糖尿病伤口愈合。
