Zuccarelli Léa, Bernard Quentin, Tarris Georges, Martin Laurent, Funes de la Vega Mathilde, Jacq Amélie, Rebibou Jean-Michel, Tinel Claire, Boccara Claude, Thouvenin Olivier, Chassot Jean-Marie, Rabant Marion, Zuber Julien, Legendre Christophe, Quenot Jean-Pierre, Peraldi Marie-Noëlle, Amrouche Lucile, Scemla Anne, Chavarot Nathalie, Anglicheau Dany, Legendre Mathieu, Maldiney Thomas
Department of Nephrology, CHU Dijon, Dijon, France.
Department of Nephrology, William Morey General Hospital, Chalon-sur-Saône, France.
Kidney Int Rep. 2025 Feb 6;10(5):1529-1537. doi: 10.1016/j.ekir.2025.01.047. eCollection 2025 May.
Kidney histology preparation requires a multistep process that is usually responsible for delayed results. This study introduces dynamic full-field optical coherence tomography (D-FF-OCT) as a label-free alternative to overcome the limitations of traditional histopathology for on-site kidney pathology assessment.
Two patient cohorts were considered, with a total of 31 patients included in the study; one cohort involved patients requiring biopsy of transplant kidney, and the other involved patients requiring biopsy of native kidney. The clinical and biological data were prospectively collected. Histopathological analysis of kidney biopsies was conducted using both conventional stains and dynamic D-FF-OCT imaging.
D-FF-OCT enabled the recognition of most kidney structures. The results showed a significant correlation between this technology and conventional stains for the evaluation of both interstitial fibrosis (IF) ( = 0.61, < 0.001) and tubular atrophy (TA) ( = 0.60, < 0.001). Although many lesions could be identified such as interstitial inflammation, acute tubular necrosis, glomerular crescents, and vascular intimal thickening; other recognitions such as glomerular membranous deposits, vascular amyloidosis, and peritubular capillaritis will require confirmation in larger cohorts.
This study demonstrates the potential of D-FF-OCT imaging for on-site analysis of kidney biopsies, providing rapid and high-resolution images without extensive sample preparation.
肾脏组织学标本制备需要多步骤流程，这通常会导致结果延迟。本研究引入动态全场光学相干断层扫描（D-FF-OCT）作为一种无标记的替代方法，以克服传统组织病理学在现场肾脏病理评估方面的局限性。
研究纳入了两个患者队列，共31例患者；一个队列包括需要对移植肾进行活检的患者，另一个队列包括需要对天然肾进行活检的患者。前瞻性收集临床和生物学数据。使用传统染色和动态D-FF-OCT成像对肾活检组织进行组织病理学分析。
D-FF-OCT能够识别大多数肾脏结构。结果显示，该技术与传统染色在评估间质纤维化（IF）（ = 0.61， < 0.001）和肾小管萎缩（TA）（ = 0.60， < 0.001）方面具有显著相关性。虽然可以识别许多病变，如间质炎症、急性肾小管坏死、肾小球新月体和血管内膜增厚；但其他识别结果，如肾小球膜性沉积物、血管淀粉样变性和肾小管周围毛细血管炎，需要在更大的队列中进行确认。
本研究证明了D-FF-OCT成像在肾活检现场分析中的潜力，可提供快速且高分辨率的图像，而无需进行广泛的样本制备。
