miR-10a是否为一种调节高级别膀胱癌增殖和侵袭的肿瘤抑制因子？

Is miR-10a a tumor suppressor that modulates proliferation and invasion in high-grade bladder cancer?

作者信息

Rodrigues THAINá, Candido PATRíCIA, Maluf Feres Camargo, ROMãO Poliana, Mioshi Carolina Mie, GUIMARãES Vanessa Ribeiro, DE Camargo Juliana Alves, DA Silva Karina Serafim, Dos Santos Gabriel Arantes, Silva Iran Amorim, Leite Katia Ramos Moreira, Nahas William C, Reis Sabrina T, Pimenta Ruan, Viana Nayara Izabel

机构信息

Laboratory of Medical Investigation (LIM55), Urology Department, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo, 01246-903, Brazil.

Department of Biology, Instituto Federal de Educação, Ciência e Tecnologia de São Paulo (IFSP), Campus São Paulo, São Paulo, 01109-010, Brazil.

出版信息

Oncol Res. 2025 May 29;33(6):1377-1382. doi: 10.32604/or.2025.055306. eCollection 2025.

DOI:10.32604/or.2025.055306

PMID:40486882

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12144657/

Abstract

OBJECTIVES

Bladder Cancer (BC) is one of the most commonly diagnosed malignancies worldwide, with high rates of mortality and morbidity. It can be classified as non-muscle invasive bladder cancer (NMIBC) or muscle-invasive bladder cancer (MIBC), with radical cystectomy being the treatment for MIBC, which significantly reduces quality of life. MicroRNAs (miRs) act as critical genetic regulators, with both oncogenic and tumor-suppressive roles. MiR-10a is described as a tumor suppressor in various neoplasms, but its role in BC is controversial. This study aims to assess the activity of miR-10a in cellular invasion and proliferation in two distinct BC cell lines.

METHODS

The study used high-grade T24 and low-grade RT4 bladder cell lines. Cells were transfected with miR-10a mimic or a non-targeting control. Transfection efficiency was validated by qPCR. Cell proliferation was cultured for 10-14 days. Cell migration and invasion were evaluated using Matrigel. All assays were conducted in triplicate.

RESULTS

The T24 cells transfected with miR-10a presented decreased cellular proliferation and invasion compared to the Scramble ( = 0.0481 and < 0.0001, respectively). In the RT4 cell line, there was only a significant reduction in cellular proliferation after miR-10a transfection ( = 0.0029). Our findings suggest that miR-10a has a tumoral suppressor role in BC, demonstrating higher efficacy in high-grade cells.

摘要

目的

膀胱癌（BC）是全球最常见的诊断恶性肿瘤之一，死亡率和发病率都很高。它可分为非肌层浸润性膀胱癌（NMIBC）或肌层浸润性膀胱癌（MIBC），根治性膀胱切除术是MIBC的治疗方法，但这会显著降低生活质量。微小RNA（miRs）作为关键的基因调节因子，具有致癌和抑癌作用。MiR-10a在各种肿瘤中被描述为一种肿瘤抑制因子，但其在BC中的作用存在争议。本研究旨在评估miR-10a在两种不同BC细胞系的细胞侵袭和增殖中的活性。

方法

本研究使用了高分级T24和低分级RT4膀胱癌细胞系。细胞用miR-10a模拟物或非靶向对照进行转染。通过qPCR验证转染效率。细胞增殖培养10 - 14天。使用基质胶评估细胞迁移和侵袭。所有实验均重复三次。

结果

与对照组相比，用miR-10a转染的T24细胞的细胞增殖和侵袭能力下降（分别为P = 0.0481和P < 0.0001）。在RT4细胞系中，miR-10a转染后仅细胞增殖有显著降低（P = 0.0029）。我们的研究结果表明，miR-10a在BC中具有肿瘤抑制作用，在高分级细胞中显示出更高的效力。