Chen Karis, Chen Yen-Chia, Yu Tung-Yang, Lin Li-Ping, Hsu Hung-Chih, Liao Yu-Cih, Su Pang Jong-Hwei, Tsai Wen-Chung
Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Linkou, Taoyuan City, Taiwan.
Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital at Taoyuan, Taoyuan City, Taiwan.
Regen Ther. 2025 May 13;30:9-19. doi: 10.1016/j.reth.2025.05.004. eCollection 2025 Dec.
Skeletal muscle injuries are prevalent in competitive sports, often disrupting elite athletes' training programs. Platelet-rich plasma (PRP) is abundant in growth factors, cytokines, and chemokines, making it a potential solution for treating muscle injuries. Adipose-derived stem cells (ADSCs) therapy presents promise for tissue regeneration, given their self-renew and differentiation capabilities. This study aims to investigate the impact of PRP on ADSCs, with a focus on cell migration. ADSCs were isolated from the subcutaneous adipose tissue of Sprague Dawley rats. Subsequently, these ADSCs were treated with PRP. The migration ability was assessed through in vitro wound assays, migration assays, and cell spreading assays. Molecular analysis, employing Western blot, was performed to examine the expression levels of phosphorylated focal adhesion kinase (FAK), FAK, phosphorylated paxillin, paxillin, and vinculin. The filamentous actin (F-actin) cytoskeleton formation was assessed through immunofluorescence staining. The study revealed a positive impact of PRP on ADSC migration in the in vitro wound assay, migration assay, and cell spreading assay. PRP elevated the expression levels of phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin in ADSCs. Additionally, PRP affected the distribution of F-actin in ADSCs. Furthermore, siRNA-mediated knockdown of FAK and paxillin confirmed their essential roles in PRP-enhanced ADSC migration. In conclusion, our findings demonstrated that PRP promoted the migration, spreading, in vitro wound healing, and cytoskeleton rearrangement of ADSCs by upregulating the expressions of phosphorylated FAK, FAK, phosphorylated paxillin, paxillin, and vinculin. This insight supports the potential use of PRP in combination with ADSCs for regeneration therapies.
骨骼肌损伤在竞技体育中很常见，常常打乱精英运动员的训练计划。富含血小板血浆（PRP）富含生长因子、细胞因子和趋化因子，使其成为治疗肌肉损伤的潜在解决方案。脂肪来源干细胞（ADSCs）疗法因其自我更新和分化能力而具有组织再生的潜力。本研究旨在探讨PRP对ADSCs的影响，重点关注细胞迁移。从Sprague Dawley大鼠的皮下脂肪组织中分离出ADSCs。随后，用PRP处理这些ADSCs。通过体外伤口试验、迁移试验和细胞铺展试验评估迁移能力。采用蛋白质免疫印迹法进行分子分析，以检测磷酸化粘着斑激酶（FAK）、FAK、磷酸化桩蛋白、桩蛋白和纽蛋白的表达水平。通过免疫荧光染色评估丝状肌动蛋白（F-肌动蛋白）细胞骨架的形成。研究表明，在体外伤口试验、迁移试验和细胞铺展试验中，PRP对ADSC迁移有积极影响。PRP提高了ADSCs中磷酸化FAK、FAK、磷酸化桩蛋白、桩蛋白和纽蛋白的表达水平。此外，PRP影响了ADSCs中F-肌动蛋白的分布。此外，siRNA介导的FAK和桩蛋白敲低证实了它们在PRP增强的ADSC迁移中的重要作用。总之，我们的研究结果表明，PRP通过上调磷酸化FAK、FAK、磷酸化桩蛋白、桩蛋白和纽蛋白的表达，促进了ADSCs的迁移、铺展、体外伤口愈合和细胞骨架重排。这一见解支持了PRP与ADSCs联合用于再生治疗的潜在用途。
