• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂肪干细胞联合富血小板血浆促进全层皮肤缺损大鼠模型的伤口愈合。

Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects.

机构信息

Department of Plastic Surgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

Department of Thyroid and Breast Surgery, the First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, China.

出版信息

Stem Cell Res Ther. 2021 Apr 6;12(1):226. doi: 10.1186/s13287-021-02257-1.

DOI:10.1186/s13287-021-02257-1
PMID:33823915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8022317/
Abstract

BACKGROUND

Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing.

METHODS

ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay.

RESULTS

ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP, and negative control. The expression levels of VEGF, p-STAT3, and SDF-1 in the ADSC+PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP.

CONCLUSIONS

PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

摘要

背景

由于糖尿病的多因素病因，患者的伤口愈合受损，这限制了各种方法的治疗效果。本研究假设脂肪来源干细胞（ADSCs）和富含血小板的血浆（PRP）的联合使用可能实现最佳有效的糖尿病伤口愈合。

方法

从 Sprague-Dawley（SD）大鼠的脂肪组织中分离 ADSCs。通过两步离心技术制备 PRP。在 SD 大鼠的背部建立糖尿病伤口模型，以评估掺入 PRP 的 ADSCs 的效果。通过苏木精和伊红染色、免疫荧光和免疫组织化学观察血管新生的变化。ELISA 和 Western blot 用于检测血管生成相关蛋白的表达水平。通过 MTS 测定评估内皮细胞的增殖。

结果

掺入 PRP 的 ADSCs 比 ADSCs、PRP 和阴性对照诱导更高的伤口闭合率。ADSC+PRP 组中 VEGF、p-STAT3 和 SDF-1 的表达水平高于其他组。此外，ADSC 条件培养基（ADSC-CM）和 PRP 的联合处理强烈刺激内皮细胞的增殖。

结论

PRP 增强了 ADSCs 诱导的糖尿病伤口愈合，其促进作用涉及血管新生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/08863453eafb/13287_2021_2257_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/2eb4b01aef52/13287_2021_2257_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/c8e6549003e5/13287_2021_2257_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/8a1c30d6cc87/13287_2021_2257_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/2d35a37c1ebb/13287_2021_2257_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/9b52c5510a1c/13287_2021_2257_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/08863453eafb/13287_2021_2257_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/2eb4b01aef52/13287_2021_2257_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/c8e6549003e5/13287_2021_2257_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/8a1c30d6cc87/13287_2021_2257_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/2d35a37c1ebb/13287_2021_2257_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/9b52c5510a1c/13287_2021_2257_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c743/8022384/08863453eafb/13287_2021_2257_Fig6_HTML.jpg

相似文献

1
Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects.脂肪干细胞联合富血小板血浆促进全层皮肤缺损大鼠模型的伤口愈合。
Stem Cell Res Ther. 2021 Apr 6;12(1):226. doi: 10.1186/s13287-021-02257-1.
2
[Effects of human adipose-derived mesenchymal stem cells and platelet-rich plasma on healing of wounds with full-thickness skin defects in mice].[人脂肪间充质干细胞和富血小板血浆对小鼠全层皮肤缺损伤口愈合的影响]
Zhonghua Shao Shang Za Zhi. 2018 Dec 20;34(12):887-894. doi: 10.3760/cma.j.issn.1009-2587.2018.12.013.
3
Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway.脂肪间充质干细胞联合富血小板血浆通过调节 Notch 通路加速糖尿病创面愈合。
Stem Cell Res Ther. 2021 Jul 13;12(1):392. doi: 10.1186/s13287-021-02454-y.
4
[Effects of hypoxia-pretreated rat adipose-derived mesenchymal stem cells conditioned medium on wound healing of rats with full-thickness defects].[缺氧预处理的大鼠脂肪间充质干细胞条件培养基对全层缺损大鼠伤口愈合的影响]
Zhonghua Shao Shang Za Zhi. 2020 Sep 20;36(9):803-812. doi: 10.3760/cma.j.cn501120-20200508-00258.
5
Adipose-derived stem cells promote diabetic wound healing via the recruitment and differentiation of endothelial progenitor cells into endothelial cells mediated by the VEGF-PLCγ-ERK pathway.脂肪干细胞通过 VEGF-PLCγ-ERK 通路募集和诱导内皮祖细胞向内皮细胞分化，促进糖尿病创面愈合。
Arch Biochem Biophys. 2020 Oct 15;692:108531. doi: 10.1016/j.abb.2020.108531. Epub 2020 Aug 1.
6
Curcumin preconditioned human adipose derived stem cells co-transplanted with platelet rich plasma improve wound healing in diabetic rats.姜黄素预处理的人脂肪来源干细胞与富血小板血浆共移植可改善糖尿病大鼠的伤口愈合。
Life Sci. 2020 Sep 15;257:118091. doi: 10.1016/j.lfs.2020.118091. Epub 2020 Jul 12.
7
Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing.艾塞那肽-4与脂肪来源干细胞联合使用可促进血管生成并改善糖尿病伤口愈合。
J Transl Med. 2017 Feb 15;15(1):35. doi: 10.1186/s12967-017-1145-4.
8
The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival.富血小板血浆和脂肪源性干细胞对新生血管形成及脂肪移植存活的影响
Aesthetic Plast Surg. 2018 Feb;42(1):1-8. doi: 10.1007/s00266-017-1062-1. Epub 2018 Jan 4.
9
Enhanced Diabetic Rat Wound Healing by Platelet-Rich Plasma Adhesion Zwitterionic Hydrogel.富血小板血浆黏附两性离子水凝胶促进糖尿病大鼠创面愈合。
Ann Plast Surg. 2024 Jan 1;92(1S Suppl 1):S2-S11. doi: 10.1097/SAP.0000000000003796.
10
HIF1α overexpression enhances diabetic wound closure in high glucose and low oxygen conditions by promoting adipose-derived stem cell paracrine function and survival.HIF1α 过表达通过促进脂肪来源干细胞旁分泌功能和存活增强高糖和低氧条件下的糖尿病创面闭合。
Stem Cell Res Ther. 2020 Apr 5;11(1):148. doi: 10.1186/s13287-020-01654-2.

引用本文的文献

1
Conditioned plasma promotes full-thickness skin defect healing in a rat model.条件血浆促进大鼠模型全层皮肤缺损愈合。
Regen Ther. 2025 Aug 18;30:629-640. doi: 10.1016/j.reth.2025.08.003. eCollection 2025 Dec.
2
Therapeutic Potential of Stem Cell-Derived Exosomes in Skin Wound Healing.干细胞来源的外泌体在皮肤伤口愈合中的治疗潜力
Biomimetics (Basel). 2025 Aug 20;10(8):546. doi: 10.3390/biomimetics10080546.
3
Wound-Healing Potential of Essential Oil: A Multi-Targeted Approach Involving Inflammation, Oxidative Stress, and Apoptosis Regulation.

本文引用的文献

1
Systematic Review-The Potential Implications of Different Platelet-Rich Plasma (PRP) Concentrations in Regenerative Medicine for Tissue Repair.系统评价-富含血小板的血浆（PRP）浓度在组织修复再生医学中的潜在影响。
Int J Mol Sci. 2020 Aug 9;21(16):5702. doi: 10.3390/ijms21165702.
2
Systematic Review: Allogenic Use of Stromal Vascular Fraction (SVF) and Decellularized Extracellular Matrices (ECM) as Advanced Therapy Medicinal Products (ATMP) in Tissue Regeneration.系统评价：基质血管成分（SVF）和去细胞细胞外基质（ECM）作为组织再生的高级治疗药物（ATMP）的同种异体应用。
Int J Mol Sci. 2020 Jul 15;21(14):4982. doi: 10.3390/ijms21144982.
3
精油的伤口愈合潜力：一种涉及炎症、氧化应激和细胞凋亡调节的多靶点方法。
Pharmaceuticals (Basel). 2025 Jun 12;18(6):880. doi: 10.3390/ph18060880.
4
Evaluation of a Kenaf Nanocrystalline Cellulose-based Hydrogel Containing Platelet Lysate for Full-thickness Wound Healing.含血小板裂解物的洋麻纳米晶纤维素基水凝胶用于全层伤口愈合的评估。
Recent Adv Drug Deliv Formul. 2025;19(1):72-85. doi: 10.2174/0126673878323868240924154455.
5
Platelet rich plasma promotes cell migration of adipose-derived stem cells by up-regulation of focal adhesion kinase and paxillin.富血小板血浆通过上调粘着斑激酶和桩蛋白促进脂肪来源干细胞的细胞迁移。
Regen Ther. 2025 May 13;30:9-19. doi: 10.1016/j.reth.2025.05.004. eCollection 2025 Dec.
6
SVF-gel application for the alleviation of full-thickness skin graft contraction: an experimental study in mice.脂肪干细胞胶用于减轻全层皮肤移植收缩：一项小鼠实验研究
Sci Rep. 2025 Apr 29;15(1):15082. doi: 10.1038/s41598-025-99649-7.
7
FG-4592 combined with PRP significantly accelerates the healing of refractory diabetic wounds by upregulating HIF-1α.FG-4592联合富血小板血浆通过上调缺氧诱导因子-1α显著加速难治性糖尿病伤口的愈合。
Sci Rep. 2025 Apr 24;15(1):14292. doi: 10.1038/s41598-025-99356-3.
8
Overexpression of miR-192 in fibroblasts accelerates wound healing in diabetic rats: research article.成纤维细胞中miR-192的过表达加速糖尿病大鼠伤口愈合：研究论文。
Eur J Med Res. 2025 Apr 4;30(1):239. doi: 10.1186/s40001-025-02449-y.
9
KLF5 enhances CXCL12 transcription in adipose-derived stem cells to promote endothelial progenitor cells neovascularization and accelerate diabetic wound healing.KLF5增强脂肪来源干细胞中CXCL12的转录，以促进内皮祖细胞的血管新生并加速糖尿病伤口愈合。
Cell Mol Biol Lett. 2025 Mar 4;30(1):24. doi: 10.1186/s11658-025-00702-0.
10
Ameliorative Effect of Combined Placenta-Derived Mesenchymal Stem Cells plus Platelet-rich Plasma on Polycystic Ovarian Model in Rats: A Biochemical and Histological Study.联合胎盘来源间充质干细胞加富血小板血浆对大鼠多囊卵巢模型的改善作用：一项生化与组织学研究
Reprod Sci. 2025 Mar;32(3):907-918. doi: 10.1007/s43032-025-01791-0. Epub 2025 Jan 24.
Adipose-derived stromal stem cells (ASCs) as a new regenerative immediate therapy combating coronavirus (COVID-19)-induced pneumonia.
脂肪来源的间充质干细胞（ASCs）作为一种新型的再生即时疗法，用于对抗冠状病毒（COVID-19）引起的肺炎。
Expert Opin Biol Ther. 2020 Jul;20(7):711-716. doi: 10.1080/14712598.2020.1761322. Epub 2020 Apr 29.
4
Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation.不同制备方法对整形美容中自体非激活富血小板血浆（A-PRP）和激活富血小板血浆（AA-PRP）的影响：伤口愈合和毛发生长评估。
Int J Mol Sci. 2020 Jan 9;21(2):431. doi: 10.3390/ijms21020431.
5
Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers.伤口愈合：基于透明质酸和富血小板血浆的生物功能化支架在慢性溃疡中的体外和体内评估
J Clin Med. 2019 Sep 18;8(9):1486. doi: 10.3390/jcm8091486.
6
Platelet-rich plasma in combination with adipose-derived stem cells promotes skin wound healing through activating Rho GTPase-mediated signaling pathway.富含血小板血浆与脂肪来源干细胞联合通过激活Rho GTPase介导的信号通路促进皮肤伤口愈合。
Am J Transl Res. 2019 Jul 15;11(7):4100-4112. eCollection 2019.
7
Concise Review: Adipose-Derived Stem Cells (ASCs) and Adipocyte-Secreted Exosomal microRNA (A-SE-miR) Modulate Cancer Growth and proMote Wound Repair.简明综述：脂肪来源干细胞（ASCs）与脂肪细胞分泌的外泌体微小RNA（A-SE-miR）对癌症生长的调节及对伤口修复的促进作用
J Clin Med. 2019 Jun 15;8(6):855. doi: 10.3390/jcm8060855.
8
Platelet-rich plasma for the treatment of diabetic foot ulcers: A meta-analysis.富血小板血浆治疗糖尿病足溃疡：一项荟萃分析。
Wound Repair Regen. 2019 Mar;27(2):170-182. doi: 10.1111/wrr.12690. Epub 2018 Dec 21.
9
Adipose-derived stem cells for wound healing.脂肪来源干细胞在伤口愈合中的应用。
J Cell Physiol. 2019 Jun;234(6):7903-7914. doi: 10.1002/jcp.27922. Epub 2018 Dec 4.
10
Wound Healing: A Cellular Perspective.创伤愈合：细胞视角。
Physiol Rev. 2019 Jan 1;99(1):665-706. doi: 10.1152/physrev.00067.2017.