Bharathi Arasangam, Ravichandran Devaraj, Saipraba Sivakumar, Balasubramanium Ravindran, Prakash Palanisamy, Gatasheh Mansour K, Ahamed Anis, Murugesan Subban
Department of Botany, Periyar University, Salem, Tamilnadu 636011 India.
Department of Biochemistry, College of Science, King Saud University, P.O. Box 11451, Riyadh, Saudi Arabia.
3 Biotech. 2025 Jul;15(7):202. doi: 10.1007/s13205-025-04338-x. Epub 2025 Jun 4.
The synthesis of iron oxide nanoparticles (FeONPs) by a recently developed green approach is very promising due to its non-toxic and environmentally friendly behavior. In this study, biosynthesis of FeONPs was prepared using leaf extract of plant with ferric chloride solution. They were characterized by UV-vis spectrum show a peak at 293 nm, confirming the presence of FeONPs. A Fourier-transform infrared (FTIR) analysis of FeONPs displays spectra that are identical to functional groups and X-ray diffraction (XRD) analysis demonstrated that the particles were of high purity with crystalline cubic structure phases in nature. The structural characteristics were determined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis which showed spherical morphology with particle diameters of between 43 nm. Energy dispersive x-ray spectroscopy (EDAX) confirmed the presence of Fe elements at 3 keV. The FeONPs showed strong antibacterial activity against Gram-positive pathogens with having the inhibition zone ranged from 19 ± 0.42 mm at a concentration of 75 μg/mL. Antioxidant activity observed more effective in assays for DPPH and ABTS radical scavenging. The anti-inflammatory activity had a stronger effect than the IC values for albumin denaturation (58.43 µg/mL) and human red blood cells (59.57 µg/mL). The FeONPs were an IC value of 48.51 μg/mL when used against breast cancer (MCF-7) cell lines. Further, according to the results of the photocatalytic activity of methylene blue (MB) dye breakdown in sunlight, MB decomposed by 85.83% after 70 min. Therefore, all of these results point to the possibility that produced FeONPs might be economical and safe for the environment when it comes to removing dyes and propose their effective applications in biologic domains in future.
最近开发的绿色方法合成氧化铁纳米颗粒（FeONPs）因其无毒且环保的特性而极具前景。在本研究中，利用植物叶片提取物与氯化铁溶液制备了FeONPs的生物合成物。通过紫外可见光谱对其进行表征，结果显示在293nm处有一个峰，证实了FeONPs的存在。对FeONPs进行的傅里叶变换红外（FTIR）分析显示出与官能团相同的光谱，X射线衍射（XRD）分析表明这些颗粒具有高纯度，本质上为结晶立方结构相。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析确定了其结构特征，结果显示为球形形态，粒径在43nm之间。能量色散X射线光谱（EDAX）证实了在3keV处存在铁元素。FeONPs对革兰氏阳性病原体表现出强大的抗菌活性，在浓度为75μg/mL时，抑菌圈范围为19±0.42mm。在DPPH和ABTS自由基清除试验中观察到抗氧化活性更有效。抗炎活性比对白蛋白变性（58.43μg/mL）和人红细胞（59.57μg/mL）的IC值具有更强的作用。当用于对抗乳腺癌（MCF-7）细胞系时，FeONPs的IC值为48.51μg/mL。此外，根据亚甲基蓝（MB）染料在阳光下光催化降解的结果，70分钟后MB分解了85.83%。因此，所有这些结果表明，所制备的FeONPs在去除染料方面可能对环境经济且安全，并有望在未来的生物领域得到有效应用。
