Wagnild J P, McDonald P, Craig W A, Johnson C, Hanley M, Uman S J, Ramgopal V, Beirne G J
Am J Med. 1977 May;62(5):672-6. doi: 10.1016/0002-9343(77)90869-5.
Blood for culture was obtained over a six week period from 17 patients undergoing long-term hemodialysis. Bacteremia was detected during 18 of 201 dialyses. Blood drawn during fifteen of these dialyses contained pseudomonas aeruginosa. Ten of the 17 patients (59 per cent) had a Pseudomonas bacteremia some time during the study. Only one patient was symptomatic. The frequency of positive cultures was related to reuse of coils. No cultures were positive until after the fifth use, but by the tenth use, 41 per cent of the dialyses were associated with bacteremia. All coils that were used repeatedly and 32 of 48 of those used only once, grew Ps. aeruginosa when filled with media and incubated. This suggests that the coils were inoculated during dialysis and that benzalkonium chloride, the sterilizing agent, was unable to eradicate this organism. With repeated uses, the number of residual bacteria in the coil became large enough to cause detectable bacteremia during dialysis.
在六周时间内，从17名接受长期血液透析的患者身上采集血样进行培养。在201次透析中有18次检测到菌血症。其中15次透析所采集的血液中含有铜绿假单胞菌。17名患者中有10名（59%）在研究期间的某个时候出现了假单胞菌菌血症。只有一名患者有症状。培养阳性的频率与透析器复用有关。在第五次使用前培养均为阴性，但到第十次使用时，41%的透析与菌血症有关。所有重复使用的透析器以及48个仅使用过一次的透析器中的32个，在填充培养基并培养后都培养出了铜绿假单胞菌。这表明透析器在透析过程中被接种，而消毒剂苯扎氯铵无法根除这种微生物。随着重复使用，透析器中残留细菌的数量变得足够多，以至于在透析期间能够检测到菌血症。
