Shekari Ahmad, Hassan Jalal, Koohi Mohammad Kazem, Ghadipasha Masoud, Akhgari Maryam
Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Forensic Toxicology, Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
MethodsX. 2025 May 8;14:103353. doi: 10.1016/j.mex.2025.103353. eCollection 2025 Jun.
This study aims to concurrently assess the blood concentrations of antiepileptic drugs including phenobarbital, carbamazepine, primidone, phenytoin in post-mortem samples through the application modified QuEChERS extraction. The serum samples were extracted and then purified using magnesium sulfate, PSA, and C18 sorbents and subsequently analyzed using HPLC/UV. One influencing parameter on the extraction process was the pH of sample. Extraction recoveries were in the range of 42-97 % for all analytes. The findings reveal that the QuEChERS method offers a higher extraction efficiency, yielding cleaner samples with greater purity and preferred choice for drug analysis in forensic toxicology. The results showed that the proposed technique has a good analytical efficiency and the accuracy of work is in the average range of 70-85 %. The equation of linear calibration curve was obtained with regression coefficient > 0.99. Detection limits were in the range of 0.21-0.38 ng/mL.•This method is inexpensive and fast for determination of antiepileptic drugs in serum•This method has high thought of sample determination.•This method is green chemistry method.
本研究旨在通过应用改良的QuEChERS提取法，同时测定死后样本中包括苯巴比妥、卡马西平、扑米酮、苯妥英在内的抗癫痫药物的血药浓度。血清样本经提取后，使用硫酸镁、PSA和C18吸附剂进行净化，随后采用HPLC/UV进行分析。提取过程中的一个影响参数是样品的pH值。所有分析物的提取回收率在42-97%范围内。研究结果表明，QuEChERS方法具有更高的提取效率，能得到纯度更高、更纯净的样品，是法医毒理学中药物分析的首选方法。结果显示，所提出的技术具有良好的分析效率，工作准确性在70-85%的平均范围内。获得了线性校准曲线方程，回归系数>0.99。检测限在0.21-0.38 ng/mL范围内。•该方法测定血清中抗癫痫药物价格低廉且快速•该方法对样品测定评价很高。•该方法是绿色化学方法。
