Hopman W P, Jansen J B, Lamers C B
Scand J Gastroenterol. 1985 Sep;20(7):843-7. doi: 10.3109/00365528509088832.
The effect of ingestion of 50 g fat, 50 g protein, and 50 g starch on plasma cholecystokinin (CCK) concentrations was studied in eight healthy volunteers. Plasma CCK concentrations were measured by radioimmunoassay with Bolton-Hunter-labelled CCK 33, CCK 33 standard, and antibody T204. Antibody T204 was directed to the sulphated tyrosine region of CCK. Ingestion of fat and protein induced significant increases in plasma CCK, whereas ingestion of starch did not significantly influence plasma CCK levels. Peak increments in plasma CCK after fat (4.8 +/- 0.9 pmol/l) and protein (3.4 +/- 0.5 pmol/l) were significantly greater than that after starch (0.9 +/- 0.3 pmol/l). Similarly, the integrated plasma CCK secretion after fat (213 +/- 49 pmol/l X 120 min) and after protein (178 +/- 53 pmol/l X 120 min) was significantly greater than that found after ingestion of starch (9 +/- 23 pmol/l X 120 min). It is concluded that, in contrast to starch, fat and protein are potent stimuli for the release of CCK.
在八名健康志愿者中研究了摄入50克脂肪、50克蛋白质和50克淀粉对血浆胆囊收缩素（CCK）浓度的影响。采用放射性免疫分析法，使用博尔顿-亨特标记的CCK 33、CCK 33标准品和抗体T204测量血浆CCK浓度。抗体T204针对CCK的硫酸化酪氨酸区域。摄入脂肪和蛋白质可使血浆CCK显著升高，而摄入淀粉对血浆CCK水平没有显著影响。脂肪（4.8±0.9皮摩尔/升）和蛋白质（3.4±0.5皮摩尔/升）摄入后血浆CCK的峰值增量显著高于淀粉摄入后（0.9±0.3皮摩尔/升）。同样，脂肪（213±49皮摩尔/升×120分钟）和蛋白质（178±53皮摩尔/升×120分钟）摄入后血浆CCK的综合分泌量显著高于淀粉摄入后（9±23皮摩尔/升×120分钟）。结论是，与淀粉不同，脂肪和蛋白质是CCK释放的有效刺激物。
