Pérez María E, Durantini Javier E, Milanesio María E, Durantini Edgardo N
Instituto para el Desarrollo Agroindustrial y de la Salud (IDAS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ruta Nacional 36 Km 601, Río Cuarto, Córdoba X5804BYA, Argentina.
Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, Río Cuartox, Córdoba X5804BYA, Argentina.
ACS Appl Bio Mater. 2025 Jul 21;8(7):6316-6325. doi: 10.1021/acsabm.5c00787. Epub 2025 Jun 9.
The rise of antibiotic-resistant pathogens has heightened the demand for innovative treatments, promoting advanced photodynamic materials for targeted microbial inactivation. Here, we report the synthesis of a series of photosensitizer nanomaterials consisting of BODIPY (BDP) dyes confined within ZIF-8, prepared via a straightforward one-pot nanoprecipitation method. The morphology of these materials was analyzed by SEM and TEM, and spectroscopic characterization confirmed the successful incorporation of BDP dyes within the porous ZIF-8 framework. Encapsulation significantly improved the ability of BDPs to generate reactive oxygen species (ROS) in aqueous media, with the brominated derivative, BrBDP@ZIF-8, exhibiting particularly high production of singlet molecular and superoxide anion radicals. The photodynamic activity of BrBDP@ZIF-8 was further demonstrated by its efficient degradation of tryptophan through a type II photoprocess. In addition, encapsulating BDPs within the ZIF-8 framework significantly enhanced their photostability compared with the free dyes. Antimicrobial assays revealed the strong potential of these nanomaterials for photoinactivation of . Under green light irradiation, BrBDP@ZIF-8 achieved a 99.999% reduction in the bacterial population in liquid suspensions after exposure to a light fluence of 5 J/cm. Fluorescence images of showed the characteristic green fluorescence of BODIPY, indicating an effective interaction with BDP@ZIF-8. Furthermore, effective eradication of contamination (>97%) was found on surfaces irradiated with only 0.9 J/cm. These findings highlight the potential of BDP-loaded ZIF-8 nanomaterials as robust and efficient photosensitizing agents for bacterial elimination, offering promising applications in the photokilling of bacterial cells and surface decontamination.
抗生素耐药性病原体的增加提高了对创新治疗方法的需求，推动了用于靶向微生物灭活的先进光动力材料的发展。在此，我们报告了一系列由限制在ZIF-8内的BODIPY（BDP）染料组成的光敏剂纳米材料的合成，该材料通过简单的一锅纳米沉淀法制备。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析了这些材料的形态，光谱表征证实了BDP染料成功地掺入了多孔ZIF-8骨架中。包封显著提高了BDP在水性介质中产生活性氧（ROS）的能力，其中溴化衍生物BrBDP@ZIF-8表现出特别高的单线态分子和超氧阴离子自由基产量。BrBDP@ZIF-8的光动力活性通过其通过II型光过程对色氨酸的有效降解进一步得到证明。此外，与游离染料相比，将BDP包封在ZIF-8骨架内显著增强了它们的光稳定性。抗菌试验揭示了这些纳米材料对[具体微生物]进行光灭活的强大潜力。在绿光照射下，BrBDP@ZIF-8在暴露于5 J/cm的光通量后，液体悬浮液中的细菌数量减少了99.999%。[具体微生物]的荧光图像显示了BODIPY的特征绿色荧光，表明与BDP@ZIF-8有有效的相互作用。此外，在仅用0.9 J/cm照射的表面上发现有效根除了[具体微生物]污染（>97%）。这些发现突出了负载BDP的ZIF-8纳米材料作为用于细菌消除的强大而有效的光敏剂的潜力，在细菌细胞的光杀伤和表面去污方面提供了有前景的应用。
