Cacciola Francesco, Marchesiello Wadir Mario Valentino, Galletta Micaela, Coppolino Carmelo, Russo Marina, Buccafuri Antonella, Fazio Alessia, Plastina Pierluigi, Quinto Maurizio, Dugo Paola, Mondello Luigi
Messina Institute of Technology c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, Former Veterinary School, University of Messina, Messina, Italy.
Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Foggia, Italy.
J Sep Sci. 2025 Jun;48(6):e70189. doi: 10.1002/jssc.70189.
The olive oil industry is currently experiencing a period of significant growth, largely as a result of the high value placed on olive oil as a high-nutritional food product on a global scale. Industrial olive oil production generates significant quantities of solid waste and dark liquid effluents, involving olive leaves (OLs), olive pomace (OP), and olive mill wastewaters (OMWWs). Particular attention was given to the recycling and valorization of by-products and biomass from the olive oil industry due to their richness in bioactive compounds, including phenolic compounds with antioxidant properties. The aim of this study was the sustainable recovery and characterization of valuable biocompounds, notably phenols, with particular emphasis on lipophenols, from different matrices (OMWWs, OP, and OLs) of three different Olea europaea cultivars using LC coupled with photodiode array (PDA) and MS detection. Method validation demonstrated satisfactory linearity (R > 0.9806), repeatability (lower than 9.82%) and recovery (over 79.07%) for all samples investigated. Before quantification, the matrix effect was also evaluated with values ranging from 71.99% to 139.70%.The results highlight that OLs, OP, and OMWWs might be employed for several applications, including nutraceuticals, cosmeceuticals, food, and animal feed.
橄榄油行业目前正经历一段显著增长的时期，这在很大程度上是由于全球范围内橄榄油作为一种高营养食品受到高度重视。工业橄榄油生产会产生大量的固体废物和深色液体废水，包括橄榄叶（OLs）、橄榄果渣（OP）和橄榄油厂废水（OMWWs）。由于橄榄油行业的副产品和生物质富含生物活性化合物，包括具有抗氧化特性的酚类化合物，因此人们特别关注它们的回收和增值利用。本研究的目的是使用液相色谱结合光电二极管阵列（PDA）和质谱检测，从三个不同油橄榄品种的不同基质（OMWWs、OP和OLs）中可持续地回收和表征有价值的生物化合物，特别是酚类化合物，尤其侧重于脂酚。方法验证表明，所有研究样品均具有令人满意的线性（R > 0.9806）、重复性（低于9.82%）和回收率（超过79.07%）。在定量之前，还评估了基质效应，其值范围为71.99%至139.70%。结果表明，OLs、OP和OMWWs可用于多种应用，包括营养保健品、药妆品、食品和动物饲料。
