Munir Asim, Lv Yuetong, Song Ruilong, Lu Xiaoyu, Li Zhichao, Wang Zhiqiang, Li Ruichao
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, PR China.
College of Pharmacy and Chemistry & Chemical Engineering, Taizhou University, Taizhou, PR China.
Vet Microbiol. 2025 Aug;307:110585. doi: 10.1016/j.vetmic.2025.110585. Epub 2025 Jun 6.
Although carbapenems have not been approved for animal use, carbapenem-resistant Enterobacteriaceae (CRE) strains are increasingly being detected in animals, posing a significant public health risk. However, the epidemiological characteristics of CRE isolates in animals remain elusive. We comprehensively investigated the genetic features of carbapenem-resistant Enterobacteriaceae isolates recovered from minks in Shandong province, China. A total of seven (38.8 %) NDM-positive isolates were identified including three Morganella morganii, three Providencia rettgeri, and one Escherichia coli from 18 samples comprises of mink feed, intestinal content, liver, spleen and lungs collected from four deceased minks. Among these isolates, only one carried the bla gene, while the remaining six harbored the bla gene and exhibited high levels of meropenem resistance (32-128 µg/mL). Genome sequencing confirmed that all M. morganii and P. rettgeri isolates harbored the chromosomally located bla gene while the E. coli isolate belonging to ST602 harbored the bla gene on ∼88 kb IncFII (pHN7A8) plasmid. To extent of our findings, this is the first discovery of carbapenem resistant Enterobacteriaceae in minks specifically the E. coli ST602 mink harboring the plasmid-mediated bla gene. In addition to E. coli, we have also reported a novel ST214 in P. rettgeri isolates harbored bla gene. Moreover, CTX-M-positive E. coli ST602 was also reported in wild birds from Chile while E. coli harboring bla gene on IncFIA/IncFII plasmid was also reported from healthy individuals in China. So, the emergence of these antimicrobial resistance genes in minks and their close association with humans and animals is alarming. Our findings highlight the urgent need for further surveillance of bacterial resistance to last-resort antibiotics in the animal settings One Health approach.
虽然碳青霉烯类药物尚未被批准用于动物，但在动物中越来越多地检测到耐碳青霉烯类肠杆菌科（CRE）菌株，这对公众健康构成了重大风险。然而，动物中CRE分离株的流行病学特征仍不清楚。我们全面调查了从中国山东省水貂中分离出的耐碳青霉烯类肠杆菌科菌株的遗传特征。从4只死亡水貂收集的18份样本（包括水貂饲料、肠道内容物、肝脏、脾脏和肺）中，共鉴定出7株（38.8%）NDM阳性分离株，包括3株摩根摩根菌、3株雷氏普罗威登斯菌和1株大肠杆菌。在这些分离株中，只有1株携带bla基因，其余6株携带bla基因并表现出对美罗培南的高水平耐药性（32 - 128μg/mL）。基因组测序证实，所有摩根摩根菌和雷氏普罗威登斯菌分离株都携带位于染色体上的bla基因，而属于ST602的大肠杆菌分离株在约88kb的IncFII（pHN7A8）质粒上携带bla基因。就我们目前的研究结果而言，这是首次在水貂中发现耐碳青霉烯类肠杆菌科细菌，特别是携带质粒介导bla基因的大肠杆菌ST602水貂。除了大肠杆菌，我们还在雷氏普罗威登斯菌分离株中报道了一种携带bla基因的新型ST214。此外，在智利的野生鸟类中也报道了CTX - M阳性的大肠杆菌ST602，在中国的健康个体中也报道了在IncFIA/IncFII质粒上携带bla基因的大肠杆菌。因此，这些抗菌药物耐药基因在水貂中的出现及其与人类和动物的密切关联令人担忧。我们的研究结果凸显了迫切需要在“同一健康”方法的动物环境中进一步监测对最后手段抗生素的细菌耐药性。
