Morgan D L, Wenzel D G
Toxicology. 1985 Aug;36(2-3):243-51. doi: 10.1016/0300-483x(85)90057-5.
We have reported a model system with which to study factors influencing the toxicity of O3 for cultured rat lung cells. This continuing investigation was to determine the relative contributions of free radical species to the toxicity of O3 as measured by 51Cr release and the use of free radical scavengers. Toxicity from modes of O3 exposure favoring stable free radicals (stationary cultures or added O3-exposed medium) was prevented by the H2O2 scavenger, catalase. Toxicity produced by exposing cells to O3 through only a thin film of medium (rotated cultures) was partially prevented by either catalase or superoxide dismutase. Their combination completely prevented toxicity, suggesting that both H2O2 and O2 were major toxic species. Mannitol, an OH scavenger formed from H2O2 and O2, was partially protective with all models while the 1O2 scavengers histidine, tryptophan and xanthine were ineffective.
我们已经报道了一个用于研究影响臭氧对培养的大鼠肺细胞毒性的因素的模型系统。这项持续的研究旨在确定自由基种类对臭氧毒性的相对贡献，通过铬-51释放以及使用自由基清除剂来衡量。过氧化氢清除剂过氧化氢酶可防止因有利于稳定自由基的臭氧暴露模式（静止培养或添加经臭氧处理的培养基）所产生的毒性。通过仅透过一层薄培养基（旋转培养）将细胞暴露于臭氧所产生的毒性，可被过氧化氢酶或超氧化物歧化酶部分阻止。它们的组合完全阻止了毒性，这表明过氧化氢和超氧阴离子都是主要的毒性物质。甘露醇是由过氧化氢和超氧阴离子形成的羟基自由基清除剂，在所有模型中都具有部分保护作用，而单线态氧清除剂组氨酸、色氨酸和黄嘌呤则无效。
