Van der Zee J, Dubbelman T M, Raap T K, Van Steveninck J
Biochem J. 1987 Mar 15;242(3):707-12. doi: 10.1042/bj2420707.
Exposure of L929 murine fibroblasts to ozone resulted in K+ leakage and inhibition of several enzymes. Most sensitive to ozone exposure were glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase. The activities of another cytosolic enzyme, lactate dehydrogenase, the mitochondrial enzymes glutamate dehydrogenase, succinate dehydrogenase, cytochrome c oxidase and the activity of the lysosomal enzymes acid phosphatase and beta-glucuronidase were, initially, not or only slightly affected. The localization of the lysosomal enzymes did not change during ozone exposure. After prolonged exposure complete deterioration of the cells was observed and all enzyme activities declined. The activity of the enzymes was also monitored during ozone exposure of a sonicated cell suspension and it was shown that all these enzymes are in fact susceptible to ozone. These observations clearly demonstrate that, besides the structure and amino acid composition of an enzyme, the localization in the cell plays an important role in its susceptibility to ozone. The intracellular levels of reduced and oxidized glutathione were affected as well. The ATP content, however, proved to be insensitive to ozone exposure.
将L929小鼠成纤维细胞暴露于臭氧中会导致钾离子泄漏并抑制多种酶。对臭氧暴露最敏感的是甘油醛-3-磷酸脱氢酶和丙酮酸激酶。另一种胞质酶乳酸脱氢酶、线粒体酶谷氨酸脱氢酶、琥珀酸脱氢酶、细胞色素c氧化酶以及溶酶体酶酸性磷酸酶和β-葡萄糖醛酸酶的活性，最初没有受到影响或仅受到轻微影响。在臭氧暴露期间，溶酶体酶的定位没有改变。长时间暴露后，观察到细胞完全退化，所有酶活性均下降。在超声处理的细胞悬液暴露于臭氧期间也监测了酶的活性，结果表明所有这些酶实际上都易受臭氧影响。这些观察结果清楚地表明，除了酶的结构和氨基酸组成外，细胞内的定位在其对臭氧的敏感性中也起着重要作用。细胞内还原型和氧化型谷胱甘肽的水平也受到了影响。然而，ATP含量被证明对臭氧暴露不敏感。
