Solovyeva Maria, Stepanov Grigory, Zhuravlev Evgenii, Kozlov Denis, Zharkov Dmitry, Dvornikova Antonina, Selishchev Dmitry
Boreskov Institute of Catalysis, Lavrentieva Ave. 5, 630090 Novosibirsk, Russian Federation.
Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave. 8, 630090 Novosibirsk, Russian Federation.
Int J Biol Macromol. 2025 Jul;318(Pt 2):145089. doi: 10.1016/j.ijbiomac.2025.145089. Epub 2025 Jun 8.
Contamination of biochemical diagnostic laboratories with impurity nucleic acids (NAs) strongly affects tests using highly sensitive microbiological methods such as polymerase chain reaction because these NAs can contaminate test samples or reagents and lead to false positive results of analysis. NAs, as biomacromolecules, remain stable on the surface of many materials for a long time, and special treatments are needed to achieve the complete degradation of NAs on the material surface. In this study, we investigated the degradation of DNA fragments, genomic DNA, ribosomal RNA, and small nuclear RNA on the UVA-irradiated surface of photoactive self-cleaning textile material prepared via the functionalization of cotton/polyester blended fabric with TiO nanoparticles using a silicon binder. Special techniques using enzymes that can repair the correct structure of NAs were employed to discover the mechanism of photocatalytic NA degradation over TiO@SiO-coated fabric. The results of this study show that the decrease in the number of NA molecules detected using PCR occurs due to the accumulation of oxidative damage to nitrogenous bases and the appearance of both single-strand and double-strand breaks in the NA backbone.
生物化学诊断实验室被杂质核酸（NAs）污染会严重影响使用聚合酶链反应等高灵敏度微生物学方法的检测，因为这些核酸会污染测试样品或试剂，导致分析结果出现假阳性。核酸作为生物大分子，能在许多材料表面长期保持稳定，需要特殊处理才能实现材料表面核酸的完全降解。在本研究中，我们研究了通过用硅粘合剂将TiO纳米颗粒对棉/聚酯混纺织物进行功能化制备的光活性自清洁纺织材料在紫外线照射表面上DNA片段、基因组DNA、核糖体RNA和小核RNA的降解情况。采用能够修复核酸正确结构的酶的特殊技术来发现TiO@SiO涂层织物上光催化核酸降解的机制。本研究结果表明，使用聚合酶链反应检测到的核酸分子数量减少是由于含氮碱基的氧化损伤积累以及核酸主链中出现单链和双链断裂所致。
