鉴定用于拟南芥和大豆基因组编辑的最佳腺嘌呤和胞嘧啶碱基编辑器。
Identification of optimal adenine and cytosine base editors for genome editing in Arabidopsis and soybean.
作者信息
Jeong Yeong Yeop, Han Jun Hee, Yu Jihyeon, Bae Sangsu, Seo Pil Joon
机构信息
Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826; Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
Department of Chemistry, Hanyang University, Seoul 04673, Korea.
出版信息
BMB Rep. 2025 Jul;58(7):288-292. doi: 10.5483/BMBRep.2024-0102.
Base editors, including adenine base editors (ABEs) and cytosine base editors (CBEs), are widely used in numerous organisms to introduce site-specific sequence modifications in genomic DNA without causing double-strand breaks (DSBs). However, these editors exhibit low editing efficiencies, particularly in dicot plants, thereby limiting their application in dicot plant genome engineering. In this study, we assessed the editing efficiencies of various base editors to identify those optimal for base editing in dicot plants. We discovered that ABE8e, an ABE variant, demonstrated superior A-to-G base editing efficiency within A5-A8 windows, and A3A/Y130F-V04, a CBE variant, exhibited the highest C-to-T base editing efficiency within C4-C15 windows in both Arabidopsis and soybean protoplasts. Overall, we recommend these two base editors as prime choices for efficient genome engineering in a range of crop plants. [BMB Reports 2025; 58(7): 288-292].
碱基编辑器，包括腺嘌呤碱基编辑器（ABEs）和胞嘧啶碱基编辑器（CBEs），被广泛应用于众多生物体中，用于在基因组DNA中引入位点特异性序列修饰，而不会导致双链断裂（DSBs）。然而，这些编辑器表现出较低的编辑效率，尤其是在双子叶植物中，从而限制了它们在双子叶植物基因组工程中的应用。在本研究中，我们评估了各种碱基编辑器的编辑效率，以确定那些最适合双子叶植物碱基编辑的编辑器。我们发现，ABE变体ABE8e在A5-A8窗口内表现出卓越的A到G碱基编辑效率，而CBE变体A3A/Y130F-V04在拟南芥和大豆原生质体的C4-C15窗口内表现出最高的C到T碱基编辑效率。总体而言，我们推荐这两种碱基编辑器作为一系列作物高效基因组工程的首选。[《BMB报告》2025年；58(7): 288-292]
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